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Journal of Bacteriology, February 2001, p. 1106-1109, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1106-1109.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Preferential Cleavage of Degradative Intermediates of rpsT mRNA by the Escherichia coli RNA Degradosome

Catherine Spickler,dagger Victoria Stronge,Dagger and George A. Mackie*

Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia, Canada V6T 1Z3

Received 16 August 2000/Accepted 8 November 2000

RNase E, the principal RNase capable of initiating mRNA decay, preferentially attacks 5'-monophosphorylated over 5'-triphosphorylated substrates. Site-specific cleavage in vitro of the rpsT mRNA by RNase H directed by chimeric 2'-O-methyl oligonucleotides was employed to create truncated RNAs which are identical to authentic degradative intermediates. The rates of cleavage of two such intermediates by RNase E in the RNA degradosome are significantly faster (2.5- to 8-fold) than that of intact RNA. This verifies the preference of RNase E for degradative intermediates and can explain the frequent "all-or-none" behavior of mRNAs during the decay process.

* Corresponding author. Mailing address: Department of Biochemistry and Molecular Biology, D. H. Copp Bldg., University of British Columbia, 2146 Health Sciences Mall, Vancouver, BC, Canada V6T 1Z3. Phone: (604) 822-2792. Fax: (604) 822-5227. E-mail: gamackie{at}interchange.ubc.ca.

dagger Present address: Département de Biochimie, Université de Montréal, Montreal, Quebec, Canada.

Dagger Present address: Department of Biochemistry, University of Toronto, Toronto, Ontario, Canada.

Journal of Bacteriology, February 2001, p. 1106-1109, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1106-1109.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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