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Journal of Bacteriology, February 2001, p. 1110-1112, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1110-1112.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Na⁺/Glutamine (Asparagine) Cotransport by Staphylococcus lugdunensis and Corynebacterium amycolatum

Rafiquel I. Sarker and Peter C. Maloney^*

Department of Physiology, Johns Hopkins Medical School, Baltimore, Maryland 21205

Received 15 September 2000/Accepted 13 November 2000

Staphylococcus lugdunensis and Corynebacterium amycolatum each have a Na⁺/glutamine cotransporter that displays an ordered reaction sequence at the extracellular surface, with sodium binding (K_m of 6.5 mM) before glutamine (K_m of 50 µM). Asparagine is low-affinity substrate (K_m  1 mM) for each system.

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^* Corresponding author. Mailing address: Department of Physiology, Johns Hopkins Medical School, 725 N. Wolfe St., Baltimore, MD 21205. Phone: (410) 955-8325. Fax: (410) 955-4438. E-mail: pmaloney{at}bs.jhmi.edu.

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Journal of Bacteriology, February 2001, p. 1110-1112, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.1110-1112.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.