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Journal of Bacteriology, February 2001, p. 928-933, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.928-933.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

p-Hydroxyphenylacetic Acid Metabolism in Pseudomonas putida F6

Kevin E. O'Connor,* Bernard Witholt, and Wouter Duetz

Institut fur Biotechnologie, ETH Honggerberg, Zurich, Switzerland

Received 7 August 2000/Accepted 7 November 2000

Pseudomonas putida F6 was found to metabolize p-hydroxyphenylacetic acid through 3,4-dihydroxyphenylacetic acid, 3,4-dihydroxymandelic acid, and 3,4-dihydroxybenzaldehyde. Cell extracts of P. putida F6 catalyze the NAD(P)H-independent hydroxylation of p-hydroxyphenylacetic acid to 3,4-dihydroxyphenylacetic acid which is further oxidized to 3,4-dihydroxymandelic acid. Oxidation and decarboxylation of the latter yields 3,4-dihydroxybenzaldehyde. A red-brown color accompanies all of the above enzyme activities and is probably due to the polymerization of quinone-like compounds. 3,4-Dihydroxybenzaldehyde is further metabolized through extradiol ring cleavage.

* Corresponding author. Present address: Department of Industrial Microbiology, Ardmore House, National University of Ireland, Belfield, Dublin 4, Ireland. Phone: 353 1 706 1307. Fax: 353 1 706 1183. E-mail: kevin.oconnor{at}ucd.ie.

Journal of Bacteriology, February 2001, p. 928-933, Vol. 183, No. 3
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.3.928-933.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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