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Journal of Bacteriology, February 2001, p. 1296-1299, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1296-1299.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Demonstration that the Group II Intron from the Clostridial Conjugative Transposon Tn5397 Undergoes Splicing In Vivo

Adam P. Roberts,1 Veit Braun,2 Christoph von Eichel-Streiber,2 and Peter Mullany1,*

Department of Microbiology, Eastman Dental Hospital for Oral Health Care Sciences, University College London, University of London, London WC1X 8LD, United Kingdom,1 and Verfügungsgebäude für Forschung und Entwicklung, Institut für Medizinische Mikrobiologie und Hygiene, Johannes-Gutenberg-Universität, 55101 Mainz, Germany2

Received 21 August 2000/Accepted 21 November 2000

Previous work has identified the conjugative transposon Tn5397 from Clostridium difficile. This element was shown to contain a group II intron. Tn5397 can be conjugatively transferred from C. difficile to Bacillus subtilis. In this work we show that the intron is spliced in both these hosts and that nonspliced RNA is also present. We constructed a mutation in the open reading frame within the intron, and this prevented splicing but did not prevent the formation of the circular form of the conjugative transposon (the likely transposition intermediate) or decrease the frequency of intergeneric transfer of Tn5397. Therefore, the intron is spliced, but splicing is not required for conjugation of Tn5397.

* Corresponding author. Mailing address: Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, University of London, 256 Gray's Inn Road, London WC1X 8LD, United Kingdom. Phone: 44 020 7915 1223. Fax: 44 020 7915 1127. E-mail: pmullany{at}eastman.ucl.ac.uk.

Journal of Bacteriology, February 2001, p. 1296-1299, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1296-1299.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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