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Journal of Bacteriology, February 2001, p. 1452-1454, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1452-1454.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

SigE Is a Chaperone for the Salmonella enterica Serovar Typhimurium Invasion Protein SigD

K. Heran Darwin, Lloyd S. Robinson, and Virginia L. Miller*

Department of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri 63110

Received 2 October 2000/Accepted 16 November 2000

SigD is translocated into eucaryotic cells by a type III secretion system. In this work, evidence that the putative chaperone SigE directly interacts with SigD is presented. A bacterial two-hybrid system demonstrated that SigE can interact with itself and SigD. In addition, SigD was specifically copurified with SigE-His6 on a nickel column.

* Corresponding author. Mailing address: Washington University School of Medicine, Department of Pediatrics, 660 S. Euclid Ave., Campus Box 8208, St. Louis, MO 63110. Phone: (314) 286-2891. Fax: (314) 286-2896. E-mail: virginia{at}borcim.wustl.edu.

Journal of Bacteriology, February 2001, p. 1452-1454, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1452-1454.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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