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Journal of Bacteriology, February 2001, p. 1459-1461, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1459-1461.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Unexpected Presence of Defective glpR Alleles in Various Strains of Escherichia coli

C. Kay Holtman, Richard Thurlkill, and Donald W. Pettigrew*

Department of Biochemistry and Biophysics and Program in Microbial Genetics and Genomics, Texas A&M University, College Station, Texas 77843-2128

Received 6 September 2000/Accepted 14 November 2000

Alleles of glpR associated with the same GlpR- phenotype produce substitutions in different conserved portions of the glycerol 3-phosphate repressor which are not part of the helix-turn-helix motif. Analysis of the effects on growth and enzyme expression show that glucose repression of glycerol utilization is not dependent on a functional repressor.

* Corresponding author. Mailing address: Department of Biochemistry and Biophysics, Texas A&M University, 2128 TAMU, College Station, TX 77843-2128. Phone: (979) 845-9621. Fax: (979) 845-9274. E-mail: dpettigrew{at}tamu.edu.

Journal of Bacteriology, February 2001, p. 1459-1461, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1459-1461.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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