Drosophila as a Model Host for Pseudomonas aeruginosa Infection

doi:10.1128/JB.183.4.1466-1471.2001

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Journal of Bacteriology, February 2001, p. 1466-1471, Vol. 183, No. 4
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.4.1466-1471.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Drosophila as a Model Host for Pseudomonas aeruginosa Infection

David A. D'Argenio, Larry A. Gallagher, Celeste A. Berg, and Colin Manoil^*

Department of Genetics, University of Washington, Seattle, Washington 98195-7360

Received 7 September 2000/Accepted 16 November 2000

Using the fruit fly Drosophila melanogaster as model host, we have identified mutants of the bacterium Pseudomonas aeruginosawith reduced virulence. Strikingly, all strains strongly impairedin fly killing also lacked twitching motility; most such strainshad a mutation in pilGHIJKL chpABCDE, a gene cluster known tobe required for twitching motility and potentially encoding asignal transduction system. The pil chp genes appear to controlthe expression of additional virulence factors, however, sincethe wild-type fly-killing phenotype of a subset of mutants isolatedon the basis of their compact colony morphology indicated thattwitching motility itself was not required for full virulencein thefly.

^* Corresponding author, Mailing address: Department of Genetics, University of Washington, 1959 NE Pacific St. $---$ J205, Box 357360,Seattle, WA 98195-7360. Phone: (206)-543-7800. Fax: (206) 543-0754.E-mail: manoil{at}u.washington.edu.

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