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Journal of Bacteriology, February 2001, p. 1476-1481, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1476-1481.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Construction and Consequences of Directed Mutations Affecting the Hemin Receptor in Pathogenic Corynebacterium Species

Michael P. Schmitt* and E. Susan Drazek

Laboratory of Bacterial Toxins, Division of Bacterial, Allergenic and Parasitic Products, Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20892

Received 15 September 2000/Accepted 26 November 2000

Genes encoding an ATP-binding cassette transporter system involved in hemin iron utilization from Corynebacterium ulcerans were cloned and characterized. The genes are homologous to a hemin transport system previously identified in Corynebacterium diphtheriae. Disruption of the hmuT gene, which encodes the putative hemin receptor, resulted in greatly reduced ability of C. ulcerans to use hemin or hemoglobin as an iron source. Inactivation of hmuT in C. diphtheriae by site-specific recombination had no effect on hemin utilization, which suggests that C. diphtheriae has an additional system for transporting hemin.

* Corresponding author. Mailing address: FDA/CBER/DBPAP, 8800 Rockville Pike, Bldg. 29, Room 108, Bethesda, MD 20892. E-mail: schmitt{at}cber.fda.gov. Phone: (301) 435-2424. Fax: (301) 402-2776.

Journal of Bacteriology, February 2001, p. 1476-1481, Vol. 183, No. 4
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.4.1476-1481.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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