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Journal of Bacteriology, March 2001, p. 1517-1523, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1517-1523.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Wide-Domain Carbon Catabolite Repressor CreA Indirectly Controls Expression of the Aspergillus nidulans xlnB Gene, Encoding the Acidic Endo-beta -(1,4)-Xylanase X24†

Margarita Orejas,* Andrew P. MacCabe, José Antonio Pérez-González, Sudeep Kumar, and Daniel Ramón

Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, 46100 Burjassot, Valencia, Spain

Received 14 August 2000/Accepted 30 November 2000

The Aspergillus nidulans xlnB gene, which encodes the acidic endo-beta -(1,4)-xylanase X24, is expressed when xylose is present as the sole carbon source and repressed in the presence of glucose. That the mutation creAd30 results in considerably elevated levels of xlnB mRNA indicates a role for the wide-domain repressor CreA in the repression of xlnB promoter (xlnBp) activity. Functional analyses of xlnBp::goxC reporter constructs show that none of the four CreA consensus target sites identified in xlnBp are functional in vivo. The CreA repressor is thus likely to exert carbon catabolite repression via an indirect mechanism rather than to influence xlnB expression by acting directly on xlnB.

* Corresponding author. Mailing address: Departamento de Biotecnología, Instituto de Agroquímica y Tecnología de Alimentos, Consejo Superior de Investigaciones Científicas, Apartado de Correos 73, 46100 Burjassot, Valencia, Spain. Phone: (34) 96 3900022. Fax: (34) 96 3636301. E-mail: morejas{at}iata.csic.es.

dagger Dedicated to the memory of our friend and colleague José Antonio Pérez-González, who died on 9 August 1997.

Journal of Bacteriology, March 2001, p. 1517-1523, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1517-1523.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Flipphi, M., van de Vondervoort, P. J. I., Ruijter, G. J. G., Visser, J., Arst, H. N. Jr., Felenbok, B. (2003). Onset of Carbon Catabolite Repression in Aspergillus nidulans. PARALLEL INVOLVEMENT OF HEXOKINASE AND GLUCOKINASE IN SUGAR SIGNALING. J. Biol. Chem. 278: 11849-11857 [Abstract] [Full Text]  


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