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Journal of Bacteriology, March 2001, p. 1568-1576, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1568-1576.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Control of hemA Expression in Rhodobacter sphaeroides 2.4.1: Effect of a Transposon Insertion in the hbdA Gene

Linda Fales, Luiza Kryszak, and Jill Zeilstra-Ryalls*

Department of Biological Sciences, Oakland University, Rochester, Michigan 48309

Received 25 September 2000/Accepted 6 December 2000

The common precursor to all tetrapyrroles is 5-aminolevulinic acid (ALA), and in Rhodobacter sphaeroides its formation occurs via the Shemin pathway. ALA synthase activity is encoded by two differentially regulated genes in R. sphaeroides 2.4.1: hemA and hemT. In our investigations of hemA regulation, we applied transposon mutagenesis under aerobic conditions, followed by a selection that identified transposon insertion mutants in which hemA expression is elevated. One of these mutants has been characterized previously (J. Zeilstra-Ryalls and S. Kaplan, J. Bacteriol. 178:985-993, 1996), and here we describe our analysis of a second mutant strain. The transposon inserted into the coding sequences of hbdA, coding for S-(+)-beta -hydroxybutyryl-coenzyme A dehydrogenase and catalyzing an NAD-dependent reaction. We provide evidence that the hbdA gene product participates in polyhydroxybutyrate (PHB) metabolism and, based on our findings, we discuss possibilities as to how defective PHB metabolism might alter the level of hemA expression.

* Corresponding author. Mailing address: Department of Biological Sciences, 374 Dodge Hall, Oakland University, Rochester, MI 48309. Phone: (248) 370-4497. Fax: (248) 370-4225. E-mail: zeilstra{at}oakland.edu.

Journal of Bacteriology, March 2001, p. 1568-1576, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1568-1576.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bruscella, P., Eraso, J. M., Roh, J. H., Kaplan, S. (2008). The Use of Chromatin Immunoprecipitation to Define PpsR Binding Activity in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 190: 6817-6828 [Abstract] [Full Text]  
  • Zeilstra-Ryalls, J. H., Schornberg, K. L. (2006). Analysis of hemF Gene Function and Expression in Rhodobacter sphaeroides 2.4.1. J. Bacteriol. 188: 801-804 [Abstract] [Full Text]  
  • Biel, A. J., Canada, K., Huang, D., Indest, K., Sullivan, K. (2002). Oxygen-Mediated Regulation of Porphobilinogen Formation in Rhodobacter capsulatus. J. Bacteriol. 184: 1685-1692 [Abstract] [Full Text]  


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