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Journal of Bacteriology, March 2001, p. 1585-1594, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1585-1594.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Nucleotide Sequence and Analysis of Conjugative Plasmid pVT745

Dominique M. Galli,1,* Jinbiao Chen,1 Karen F. Novak,2 and Donald J. Leblanc1,3

School of Dentistry, Indiana University, Indianapolis, Indiana 462021; School of Dental Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania 152612; and Lilly Research Laboratories, Eli Lilly and Co., Indianapolis, Indiana 462853

Received 18 September 2000/Accepted 13 December 2000

The complete nucleotide sequence and genetic map of pVT745 are presented. The 25-kb plasmid was isolated from Actinobacillus actinomycetemcomitans, a periodontal pathogen. Two-thirds of the plasmid encode functions related to conjugation, replication, and replicon stability. Among potential gene products with a high degree of similarity to known proteins are those associated with plasmid conjugation. It was shown that pVT745 derivatives not only mobilized a coresident nontransmissible plasmid, pMMB67, but also mediated their own conjugative transfer to different A. actinomycetemcomitans strains. However, transfer of pVT745 derivatives from A. actinomycetemcomitans to Escherichia coli JM109 by conjugation was successful only when an E. coli origin of replication was present on the pVT745 construct. Surprisingly, 16 open reading frames encode products of unknown function. The plasmid contains a conserved replication region which belongs to the HAP (Haemophilus-Actinobacillus-Pasteurella) theta replicon family. However, its host range appears to be rather narrow compared to other members of this family. Sequences homologous to pVT745 have previously been detected in the chromosomes of numerous A. actinomycetemcomitans strains. The nature and origin of these homologs are discussed based on information derived from the nucleotide sequence.

* Corresponding author. Mailing address: Indiana University, School of Dentistry, Department of Oral Biology, 1121 W. Michigan St., Indianapolis, IN 46202. Phone: (317) 278-1936. Fax: (317) 278-1411. E-mail: dgalli{at}iupui.edu.

Journal of Bacteriology, March 2001, p. 1585-1594, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1585-1594.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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