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Journal of Bacteriology, March 2001, p. 1672-1679, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1672-1679.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

S-Layer Variation in Bacillus stearothermophilus PV72 Is Based on DNA Rearrangements between the Chromosome and the Naturally Occurring Megaplasmids

Holger C. Scholz,1,* Eva Riedmann,2 Angela Witte,2 Werner Lubitz,2 and Beatrix Kuen2

Institute of Microbiology and Genetics, University of Vienna, 1030 Vienna, Austria,2 and Institute of Animal Hygiene and Public Veterinary Health, 04103 Leipzig, Germany1

Received 24 August 2000/Accepted 14 November 2000

Bacillus stearothermophilus PV72 expresses different S-layer genes (sbsA and sbsB) under different growth conditions. No stretches of significant sequence identity between sbsA and sbsB were detected. In order to investigate S-layer gene regulation in B. stearothermophilus PV72, we characterized the upstream regulatory region of sbsA and sbsB by sequencing and primer extension analysis. Both genes are transcribed from unique but different promoters, independently of the growth phase. Localization of sbsB in the sbsA-expressing strain PV72/p6 revealed that the coding region of the second S-layer gene sbsB is located not on the chromosome but on a natural megaplasmid of the strain, whereas the upstream regulatory region of sbsB was exclusively detected on the chromosome of PV72/p6. For sbsB expression, the coding region has to be integrated into the chromosomally located expression site. After the switch to sbsB expression, the sbsA coding region was removed from the chromosome but could still be detected on the plasmid of the sbsB-expressing strain PV72/p2. The sbsA upstream regulatory region, however, remained on the chromosome. This is the first report of S-layer variation not caused by intrachromosomal DNA rearrangements, but where variant formation depends on recombinational events between the plasmid and the chromosome.

* Corresponding author. Mailing address: Institute of Animal Hygiene and Public Veterinary Health, An den Tierkliniken 17, 04103 Leipzig, Germany. Phone: 49-341-9738165. Fax: 49-341-9738198. E-mail: scholz{at}vetmed.uni-leipzig.de.

Journal of Bacteriology, March 2001, p. 1672-1679, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1672-1679.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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