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Journal of Bacteriology, March 2001, p. 1680-1687, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1680-1687.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

The Hook Gene (flgE) Is Expressed from the flgBCDEF Operon in Rhodobacter sphaeroides: Study of an flgE Mutant

Teresa Ballado,1 Laura Camarena,2 Bertha González-Pedrajo,1 Eugenia Silva-Herzog,1 and Georges Dreyfus1,*

Departamento de Genética Molecular, Instituto de Fisiología Celular,1 and Departamento de Biología Molecular, Instituto de Investigaciones Biomédicas,2 UNAM, 04510 México D.F., México

Received 5 October 2000/Accepted 13 December 2000

In this work we identified the flgE gene encoding the flagellar hook protein from Rhodobacter sphaeroides. Our results show that this gene is part of a flagellar cluster that includes the genes flgB, flgC, flgD, flgE, and flgF. Two different types of mutants in the flgE gene were isolated, and both showed a Fla- phenotype, indicating the functionality of this sequence. Complementation studies of these mutant strains suggest that flgE is included in a single transcriptional unit that starts in flgB and ends in flgF. In agreement with this possibility, a specific transcript of approximately 3.5 kb was identified by Northern blot. This mRNA is large enough to represent the complete flgBCDEF operon. FlgE showed a relatively high proline content; in particular, a region of 12 amino acids near the N terminus, in which four prolines were identified. Cells expressing a mutant FlgE protein lacking this region showed abnormal swimming behavior, and their hooks were curved. These results suggest that this region is involved in the characteristic quaternary structure of the hook of R. sphaeroides and also imply that a straight hook, or perhaps the rigidity associated with this feature, is important for an efficient swimming behavior in this bacterium.

* Corresponding author. Mailing address: Departamento de Genética Molecular, Instituto de Fisiología Celular, UNAM, Apdo. Postal 70-243, México D.F., México. Phone: (525) 622-56-03. Fax: (525) 616-22-82. E-mail: gdreyfus{at}ifisiol.unam.mx.

Journal of Bacteriology, March 2001, p. 1680-1687, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1680-1687.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Poggio, S., Abreu-Goodger, C., Fabela, S., Osorio, A., Dreyfus, G., Vinuesa, P., Camarena, L. (2007). A Complete Set of Flagellar Genes Acquired by Horizontal Transfer Coexists with the Endogenous Flagellar System in Rhodobacter sphaeroides. J. Bacteriol. 189: 3208-3216 [Abstract] [Full Text]  
  • Lee, H. J., Hughes, K. T. (2006). Posttranscriptional Control of the Salmonella enterica Flagellar Hook Protein FlgE. J. Bacteriol. 188: 3308-3316 [Abstract] [Full Text]  
  • Kobayashi, K., Saitoh, T., Shah, D. S. H., Ohnishi, K., Goodfellow, I. G., Sockett, R. E., Aizawa, S.-I. (2003). Purification and Characterization of the Flagellar Basal Body of Rhodobacter sphaeroides. J. Bacteriol. 185: 5295-5300 [Abstract] [Full Text]  


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