This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sáez, L. P.
Right arrow Articles by Castillo, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sáez, L. P.
Right arrow Articles by Castillo, F.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2001, p. 1780-1783, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1780-1783.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Role for draTG and rnf Genes in Reduction of 2,4-Dinitrophenol by Rhodobacter capsulatus

Lara P. Sáez,1 Patricia García,1 Manuel Martínez-Luque,1 Werner Klipp,2 Rafael Blasco,1,* and Francisco Castillo1

Departamento de Bioquímica y Biología Molecular, Universidad de Córdoba, 14071 Córdoba, Spain,1 and Lehrstuhl für Biologie der Mikroorganismen, Fakultät für Biologie, Universität Bochum, D-44780 Bochum, Germany2

Received 28 July 2000/Accepted 2 December 2000

The phototrophic bacterium Rhodobacter capsulatus is able to reduce 2,4-dinitrophenol (DNP) to 2-amino-4-nitrophenol enzymatically and thus can grow in the presence of this uncoupler. DNP reduction was switched off by glutamine or ammonium, but this short-term regulation did not take place in a draTG deletion mutant. Nevertheless, the target of DraTG does not seem to be the nitrophenol reductase itself since the ammonium shock did not inactivate the enzyme. In addition to this short-term regulation, ammonium or glutamine repressed the DNP reduction system. Mutants of R. capsulatus affected in ntrC or rpoN exhibited a 10-fold decrease in nitroreductase activity in vitro but almost no DNP activity in vivo. In addition, mutants affected in rnfA or rnfC, which are also under NtrC control and encode components involved in electron transfer to nitrogenase, were unable to metabolize DNP. These results indicate that NtrC regulates dinitrophenol reduction in R. capsulatus, either directly or indirectly, by controlling expression of the Rnf proteins. Therefore, the Rnf complex seems to supply electrons for both nitrogen fixation and DNP reduction.

* Corresponding author. Mailing address: Departamento de Bioquímica y Biología Molecular, Edificio C6, 1a Planta, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain. Phone: 34 957218318. Fax: 34 957218592. E-mail: bb1blplr{at}uco.es.

Journal of Bacteriology, March 2001, p. 1780-1783, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1780-1783.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Perez-Reinado, E., Blasco, R., Castillo, F., Moreno-Vivian, C., Roldan, M. D. (2005). Regulation and Characterization of Two Nitroreductase Genes, nprA and nprB, of Rhodobacter capsulatus. Appl. Environ. Microbiol. 71: 7643-7649 [Abstract] [Full Text]  
  • Desnoues, N., Lin, M., Guo, X., Ma, L., Carreno-Lopez, R., Elmerich, C. (2003). Nitrogen fixation genetics and regulation in a Pseudomonas stutzeri strain associated with rice. Microbiology 149: 2251-2262 [Abstract] [Full Text]  
  • Hase, C. C., Fedorova, N. D., Galperin, M. Y., Dibrov, P. A. (2001). Sodium Ion Cycle in Bacterial Pathogens: Evidence from Cross-Genome Comparisons. Microbiol. Mol. Biol. Rev. 65: 353-370 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»