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Journal of Bacteriology, March 2001, p. 1792-1795, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1792-1795.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

New Host-Vector System for Thermus spp. Based on the Malate Dehydrogenase Gene

Kevin J. Kayser* and John J. Kilbane II

Gas Technology Institute, Des Plaines, Illinois 60018

Received 11 July 2000/Accepted 28 November 2000

A Thermus thermophilus HB27 strain was constructed in which the malate dehydrogenase (mdh) gene was deleted. The Delta mdh colonies are recognized by a small-colony phenotype. Wild-type phenotype is restored by transformation with Thermus plasmids or integration vector containing an intact mdh gene. The wild-type phenotype provides a positive selection tool for the introduction of plasmid DNA into Thermus spp., and because mdh levels can be readily quantified, this host-vector system is a convenient tool for monitoring gene expression.

* Corresponding author. Mailing address: Gas Technology Institute, 1700 S. Mount Prospect Rd., Des Plaines, IL 60018-1804. Phone: (847) 768-0686. Fax: (847) 768-0669. E-mail: kayserkj{at}igt.org

Journal of Bacteriology, March 2001, p. 1792-1795, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1792-1795.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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