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Journal of Bacteriology, March 2001, p. 1801-1804, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1801-1804.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Constitutive Expression of Escherichia coli tat Genes Indicates an Important Role for the Twin-Arginine Translocase during Aerobic and Anaerobic Growth

Rachael L. Jack,1,2 Frank Sargent,1,2 Ben C. Berks,1 Gary Sawers,2 and Tracy Palmer1,2,*

Centre for Metalloprotein Spectroscopy and Biology, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ,1 and Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH,2 United Kingdom

Received 7 September 2000/Accepted 30 November 2000

The transcription start sites for the tatABCD and tatE loci, encoding components of the Tat (twin-arginine translocase) protein export pathway, have been identified. Expression studies indicate that the tatABCD and tatE transcription units are expressed constitutively. Translational fusion experiments suggest that TatA is synthesized at a much higher level than the other Tat proteins.


* Corresponding author. Mailing address: Department of Molecular Microbiology, John Innes Centre, Norwich NR4 7UH, United Kingdom. Phone: 44 (0)1603 450726. Fax: 44 (0)1603 450018. E-mail: tracy.palmer{at}bbsrc.ac.uk.


Journal of Bacteriology, March 2001, p. 1801-1804, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1801-1804.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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Copyright © 2001 by the American Society for Microbiology. All rights reserved.