Journal of Bacteriology, March 2001, p. 1813-1818, Vol. 183, No. 5
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.5.1813-1818.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Department of Microbiology, The Ohio State University, Columbus, Ohio 43210
Received 18 October 2000/Accepted 6 December 2000
In the archaeon Methanobacterium thermoautotrophicum,
MTH1669 encodes a protein with a sequence related to the N-terminal sequences of the
-subunits of eucaryal general transcription factor
TFIIE. The recombinant MTH1669 gene product has been purified and shown
to stimulate transcription in vitro from M. thermoautotrophicum promoters that were almost inactive or
much less active in reaction mixtures that contained only M. thermoautotrophicum RNA polymerase, TATA-binding protein and
transcription factor B. As all complete archaeal genome sequences
contain an MTH1669 homolog, the protein encoded by this gene is
apparently the first characterized example of a transcription
activator, here designated TFE, that may be universally present in the
Archaea.
This article has been cited by other articles:
| Appl. Environ. Microbiol. | Infect. Immun. | Eukaryot. Cell |
|---|---|---|
| Mol. Cell. Biol. | J. Virol. | Microbiol. Mol. Biol. Rev. |
| ALL ASM JOURNALS |