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Journal of Bacteriology, March 2001, p. 1813-1818, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1813-1818.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

TFE, an Archaeal Transcription Factor in Methanobacterium thermoautotrophicum Related to Eucaryal Transcription Factor TFIIEalpha

Brian L. Hanzelka, Trevor J. Darcy, and John N. Reeve*

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210

Received 18 October 2000/Accepted 6 December 2000

In the archaeon Methanobacterium thermoautotrophicum, MTH1669 encodes a protein with a sequence related to the N-terminal sequences of the alpha -subunits of eucaryal general transcription factor TFIIE. The recombinant MTH1669 gene product has been purified and shown to stimulate transcription in vitro from M. thermoautotrophicum promoters that were almost inactive or much less active in reaction mixtures that contained only M. thermoautotrophicum RNA polymerase, TATA-binding protein and transcription factor B. As all complete archaeal genome sequences contain an MTH1669 homolog, the protein encoded by this gene is apparently the first characterized example of a transcription activator, here designated TFE, that may be universally present in the Archaea.

* Corresponding author. Mailing address: Department of Microbiology, The Ohio State University, Columbus, OH 43210. Phone: (614) 292-2301. Fax: (614) 292-8120. E-mail: reeve.2{at}osu.edu.

Journal of Bacteriology, March 2001, p. 1813-1818, Vol. 183, No. 5
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.5.1813-1818.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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