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Journal of Bacteriology, March 2001, p. 1928-1937, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.1928-1937.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Complete Genomic Sequence of the Lytic Bacteriophage phi YeO3-12 of Yersinia enterocolitica Serotype O:3

Maria I. Pajunen,* Saija J. Kiljunen, M. E.-Lotta Söderholm, and Mikael Skurnik

Department of Medical Biochemistry, Institute of Biomedicine, University of Turku, Turku, Finland

Received 11 August 2000/Accepted 12 December 2000

phi YeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the phi YeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted phi YeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, phi YeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonnei D2371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik, J. Bacteriol. 182:5114-5120, 2000). These findings indicate that phi YeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.

* Corresponding author. Mailing address: Department of Medical Biochemistry, University of Turku, Kiinamyllynkatu 10, 20520 Turku, Finland. Phone: 358-2-3337444. Fax: 358-2-3337229. E-mail: maria.pajunen{at}utu.fi.

Journal of Bacteriology, March 2001, p. 1928-1937, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.1928-1937.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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