This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sledjeski, D. D.
Right arrow Articles by Zhang, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sledjeski, D. D.
Right arrow Articles by Zhang, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, March 2001, p. 1997-2005, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.1997-2005.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Hfq Is Necessary for Regulation by the Untranslated RNA DsrA

Darren D. Sledjeski,1,* Christina Whitman,1,dagger and Aixia Zhang2

Department of Microbiology and Immunology, Medical College of Ohio, Toledo, Ohio, 43614,1 and Cell Biology and Metabolism Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 208922

Received 16 August 2000/Accepted 8 January 2001

DsrA is an 85-nucleotide, untranslated RNA that has multiple regulatory activities at 30°C. These activities include the translational regulation of RpoS and H-NS, global transcriptional regulators in Escherichia coli. Hfq is an E. coli protein necessary for the in vitro and in vivo replication of the RNA phage Qbeta . Hfq also plays a role in the degradation of numerous RNA transcripts. Here we show that an hfq mutant strain is defective for DsrA-mediated regulation of both rpoS and hns. The defect in rpoS expression can be partially overcome by overexpression of DsrA. Hfq does not regulate the transcription of DsrA, and DsrA does not alter the accumulation of Hfq. However, in an hfq mutant, chromosome-expressed DsrA was unstable (half-life of 1 min) and truncated at the 3' end. When expressed from a multicopy plasmid, DsrA was stable in both wild-type and hfq mutant strains, but it had only partial activity in the hfq mutant strain. Purified Hfq binds DsrA in vitro. These results suggest that Hfq acts as a protein cofactor for the regulatory activities of DsrA by either altering the structure of DsrA or forming an active RNA-protein complex.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, Medical College of Ohio, 3055 Arlington Ave., Toledo, OH 43614-5806. Phone: (419) 383-5192. Fax: (419) 383-3002. E-mail: dsledjeski{at}mco.edu.

dagger Present address: Bio-Rad Laboratories, Life Science Group, Hercules, CA 94547.

Journal of Bacteriology, March 2001, p. 1997-2005, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.1997-2005.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Cheng, R., Miao, C., Gong, Q., Gu, Y., Lu, X., Han, F., Yu, W. (2009). Specific gene silencing by artificial trans-encoded small noncoding RNAs in bacteria. Nucleic Acids Res 0: gkp447v1-gkp447 [Abstract] [Full Text]  
  • Pulvermacher, S. C., Stauffer, L. T., Stauffer, G. V. (2009). Role of the sRNA GcvB in regulation of cycA in Escherichia coli. Microbiology 155: 106-114 [Abstract] [Full Text]  
  • Pulvermacher, S. C., Stauffer, L. T., Stauffer, G. V. (2009). Role of the Escherichia coli Hfq protein in GcvB regulation of oppA and dppA mRNAs. Microbiology 155: 115-123 [Abstract] [Full Text]  
  • Pulvermacher, S. C., Stauffer, L. T., Stauffer, G. V. (2009). The Small RNA GcvB Regulates sstT mRNA Expression in Escherichia coli. J. Bacteriol. 191: 238-248 [Abstract] [Full Text]  
  • Gorke, B., Vogel, J. (2008). Noncoding RNA control of the making and breaking of sugars. Genes Dev. 22: 2914-2925 [Abstract] [Full Text]  
  • Dienst, D., Duhring, U., Mollenkopf, H.-J., Vogel, J., Golecki, J., Hess, W. R., Wilde, A. (2008). The cyanobacterial homologue of the RNA chaperone Hfq is essential for motility of Synechocystis sp. PCC 6803. Microbiology 154: 3134-3143 [Abstract] [Full Text]  
  • Sonnleitner, E., Sorger-Domenigg, T., Madej, M. J., Findeiss, S., Hackermuller, J., Huttenhofer, A., Stadler, P. F., Blasi, U., Moll, I. (2008). Detection of small RNAs in Pseudomonas aeruginosa by RNomics and structure-based bioinformatic tools. Microbiology 154: 3175-3187 [Abstract] [Full Text]  
  • Soper, T. J., Woodson, S. A. (2008). The rpoS mRNA leader recruits Hfq to facilitate annealing with DsrA sRNA. RNA 14: 1907-1917 [Abstract] [Full Text]  
  • Kulesus, R. R., Diaz-Perez, K., Slechta, E. S., Eto, D. S., Mulvey, M. A. (2008). Impact of the RNA Chaperone Hfq on the Fitness and Virulence Potential of Uropathogenic Escherichia coli. Infect. Immun. 76: 3019-3026 [Abstract] [Full Text]  
  • Lee, T., Feig, A. L. (2008). The RNA binding protein Hfq interacts specifically with tRNAs. RNA 14: 514-523 [Abstract] [Full Text]  
  • Vecerek, B., Rajkowitsch, L., Sonnleitner, E., Schroeder, R., Blasi, U. (2008). The C-terminal domain of Escherichia coli Hfq is required for regulation. Nucleic Acids Res 36: 133-143 [Abstract] [Full Text]  
  • Lucchetti-Miganeh, C., Burrowes, E., Baysse, C., Ermel, G. (2008). The post-transcriptional regulator CsrA plays a central role in the adaptation of bacterial pathogens to different stages of infection in animal hosts. Microbiology 154: 16-29 [Abstract] [Full Text]  
  • Wadler, C. S., Vanderpool, C. K. (2007). From the Cover: A dual function for a bacterial small RNA: SgrS performs base pairing-dependent regulation and encodes a functional polypeptide. Proc. Natl. Acad. Sci. USA 104: 20454-20459 [Abstract] [Full Text]  
  • Li, J., Attila, C., Wang, L., Wood, T. K., Valdes, J. J., Bentley, W. E. (2007). Quorum Sensing in Escherichia coli Is Signaled by AI-2/LsrR: Effects on Small RNA and Biofilm Architecture. J. Bacteriol. 189: 6011-6020 [Abstract] [Full Text]  
  • Guisbert, E., Rhodius, V. A., Ahuja, N., Witkin, E., Gross, C. A. (2007). Hfq Modulates the {sigma}E-Mediated Envelope Stress Response and the {sigma}32-Mediated Cytoplasmic Stress Response in Escherichia coli. J. Bacteriol. 189: 1963-1973 [Abstract] [Full Text]  
  • Arluison, V., Hohng, S., Roy, R., Pellegrini, O., Regnier, P., Ha, T. (2007). Spectroscopic observation of RNA chaperone activities of Hfq in post-transcriptional regulation by a small non-coding RNA. Nucleic Acids Res 35: 999-1006 [Abstract] [Full Text]  
  • Urban, J. H., Vogel, J. (2007). Translational control and target recognition by Escherichia coli small RNAs in vivo. Nucleic Acids Res 35: 1018-1037 [Abstract] [Full Text]  
  • Schurdell, M. S., Woodbury, G. M., McCleary, W. R. (2007). Genetic Evidence Suggests that the Intergenic Region between pstA and pstB Plays a Role in the Regulation of rpoS Translation during Phosphate Limitation. J. Bacteriol. 189: 1150-1153 [Abstract] [Full Text]  
  • Jones, A. M., Goodwill, A., Elliott, T. (2006). Limited Role for the DsrA and RprA Regulatory RNAs in rpoS Regulation in Salmonella enterica.. J. Bacteriol. 188: 5077-5088 [Abstract] [Full Text]  
  • Rolle, K., Zywicki, M., Wyszko, E., Barciszewska, M. Z., Barciszewski, J. (2006). Evaluation of the Dynamic Structure of DsrA RNA from E. coli and Its Functional Consequences.. J Biochem 139: 431-438 [Abstract] [Full Text]  
  • Ziolkowska, K., Derreumaux, P., Folichon, M., Pellegrini, O., Regnier, P., Boni, I. V., Hajnsdorf, E. (2006). Hfq variant with altered RNA binding functions. Nucleic Acids Res 34: 709-720 [Abstract] [Full Text]  
  • GOTTESMAN, S., McCULLEN, C.A., GUILLIER, M., VANDERPOOL, C.K., MAJDALANI, N., BENHAMMOU, J., THOMPSON, K.M., FitzGERALD, P.C., SOWA, N.A., FitzGERALD, D.J. (2006). Small RNA Regulators and the Bacterial Response to Stress. Cold Spring Harb Symp Quant Biol 71: 1-11 [Abstract]  
  • Will, W. R., Frost, L. S. (2006). Hfq Is a Regulator of F-Plasmid TraJ and TraM Synthesis in Escherichia coli. J. Bacteriol. 188: 124-131 [Abstract] [Full Text]  
  • Deana, A., Belasco, J. G. (2005). Lost in translation: the influence of ribosomes on bacterial mRNA decay. Genes Dev. 19: 2526-2533 [Abstract] [Full Text]  
  • Takada, A., Nagai, K., Wachi, M. (2005). A decreased level of FtsZ is responsible for inviability of RNase E-deficient cells. GENES CELLS 10: 733-741 [Abstract] [Full Text]  
  • Davis, B. M., Quinones, M., Pratt, J., Ding, Y., Waldor, M. K. (2005). Characterization of the Small Untranslated RNA RyhB and Its Regulon in Vibrio cholerae. J. Bacteriol. 187: 4005-4014 [Abstract] [Full Text]  
  • Rediers, H., Rainey, P. B., Vanderleyden, J., De Mot, R. (2005). Unraveling the Secret Lives of Bacteria: Use of In Vivo Expression Technology and Differential Fluorescence Induction Promoter Traps as Tools for Exploring Niche-Specific Gene Expression. Microbiol. Mol. Biol. Rev. 69: 217-261 [Abstract] [Full Text]  
  • VECEREK, B., MOLL, I., BLASI, U. (2005). Translational autocontrol of the Escherichia coli hfq RNA chaperone gene. RNA 11: 976-984 [Abstract] [Full Text]  
  • Ledeboer, N. A., Jones, B. D. (2005). Exopolysaccharide Sugars Contribute to Biofilm Formation by Salmonella enterica Serovar Typhimurium on HEp-2 Cells and Chicken Intestinal Epithelium. J. Bacteriol. 187: 3214-3226 [Abstract] [Full Text]  
  • Afonyushkin, T., Vecerek, B., Moll, I., Blasi, U., Kaberdin, V. R. (2005). Both RNase E and RNase III control the stability of sodB mRNA upon translational inhibition by the small regulatory RNA RyhB. Nucleic Acids Res 33: 1678-1689 [Abstract] [Full Text]  
  • Kawamoto, H., Morita, T., Shimizu, A., Inada, T., Aiba, H. (2005). Implication of membrane localization of target mRNA in the action of a small RNA: mechanism of post-transcriptional regulation of glucose transporter in Escherichia coli. Genes Dev. 19: 328-338 [Abstract] [Full Text]  
  • Lease, R. A., Smith, D., McDonough, K., Belfort, M. (2004). The Small Noncoding DsrA RNA Is an Acid Resistance Regulator in Escherichia coli. J. Bacteriol. 186: 6179-6185 [Abstract] [Full Text]  
  • Dole, S., Klingen, Y., Nagarajavel, V., Schnetz, K. (2004). The Protease Lon and the RNA-Binding Protein Hfq Reduce Silencing of the Escherichia coli bgl Operon by H-NS. J. Bacteriol. 186: 2708-2716 [Abstract] [Full Text]  
  • Kaldalu, N., Mei, R., Lewis, K. (2004). Killing by Ampicillin and Ofloxacin Induces Overlapping Changes in Escherichia coli Transcription Profile. Antimicrob. Agents Chemother. 48: 890-896 [Abstract] [Full Text]  
  • Folichon, M., Arluison, V., Pellegrini, O., Huntzinger, E., Regnier, P., Hajnsdorf, E. (2003). The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation. Nucleic Acids Res 31: 7302-7310 [Abstract] [Full Text]  
  • MOLL, I., AFONYUSHKIN, T., VYTVYTSKA, O., KABERDIN, V. R., BLASI, U. (2003). Coincident Hfq binding and RNase E cleavage sites on mRNA and small regulatory RNAs. RNA 9: 1308-1314 [Abstract] [Full Text]  
  • Chen, G., Patten, C. L., Schellhorn, H. E. (2003). Controlled Expression of an rpoS Antisense RNA Can Inhibit RpoS Function in Escherichia coli. Antimicrob. Agents Chemother. 47: 3485-3493 [Abstract] [Full Text]  
  • Rediers, H., Bonnecarrere, V., Rainey, P. B., Hamonts, K., Vanderleyden, J., De Mot, R. (2003). Development and Application of a dapB-Based In Vivo Expression Technology System To Study Colonization of Rice by the Endophytic Nitrogen-Fixing Bacterium Pseudomonas stutzeri A15. Appl. Environ. Microbiol. 69: 6864-6874 [Abstract] [Full Text]  
  • Ruiz, N., Silhavy, T. J. (2003). Constitutive Activation of the Escherichia coli Pho Regulon Upregulates rpoS Translation in an Hfq-Dependent Fashion. J. Bacteriol. 185: 5984-5992 [Abstract] [Full Text]  
  • Masse, E., Escorcia, F. E., Gottesman, S. (2003). Coupled degradation of a small regulatory RNA and its mRNA targets in Escherichia coli. Genes Dev. 17: 2374-2383 [Abstract] [Full Text]  
  • Ahlquist, P., Noueiry, A. O., Lee, W.-M., Kushner, D. B., Dye, B. T. (2003). Host Factors in Positive-Strand RNA Virus Genome Replication. J. Virol. 77: 8181-8186 [Full Text]  
  • Sauter, C., Basquin, J., Suck, D. (2003). Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli. Nucleic Acids Res 31: 4091-4098 [Abstract] [Full Text]  
  • Worhunsky, D. J., Godek, K., Litsch, S., Schlax, P. J. (2003). Interactions of the Non-coding RNA DsrA and RpoS mRNA with the 30 S Ribosomal Subunit. J. Biol. Chem. 278: 15815-15824 [Abstract] [Full Text]  
  • BRESCIA, C. C., MIKULECKY, P. J., FEIG, A. L., SLEDJESKI, D. D. (2003). Identification of the Hfq-binding site on DsrA RNA: Hfq binds without altering DsrA secondary structure. RNA 9: 33-43 [Abstract] [Full Text]  
  • Gottesman, S. (2002). Stealth regulation: biological circuits with small RNA switches. Genes Dev. 16: 2829-2842 [Full Text]  
  • Hengge-Aronis, R. (2002). Signal Transduction and Regulatory Mechanisms Involved in Control of the {sigma}S (RpoS) Subunit of RNA Polymerase. Microbiol. Mol. Biol. Rev. 66: 373-395 [Abstract] [Full Text]  
  • Sun, X., Zhulin, I., Wartell, R. M. (2002). Predicted structure and phyletic distribution of the RNA-binding protein Hfq. Nucleic Acids Res 30: 3662-3671 [Abstract] [Full Text]  
  • Brown, L., Gentry, D., Elliott, T., Cashel, M. (2002). DksA Affects ppGpp Induction of RpoS at a Translational Level. J. Bacteriol. 184: 4455-4465 [Abstract] [Full Text]  
  • Moller, T., Franch, T., Udesen, C., Gerdes, K., Valentin-Hansen, P. (2002). Spot 42 RNA mediates discoordinate expression of the E. coli galactose operon. Genes Dev. 16: 1696-1706 [Abstract] [Full Text]  
  • Rajkumari, K., Gowrishankar, J. (2002). An N-Terminally Truncated RpoS ({sigma}S) Protein in Escherichia coli Is Active In Vivo and Exhibits Normal Environmental Regulation Even in the Absence of rpoS Transcriptional and Translational Control Signals. J. Bacteriol. 184: 3167-3175 [Abstract] [Full Text]  
  • Masse, E., Gottesman, S. (2002). A small RNA regulates the expression of genes involved in iron metabolism in Escherichiacoli. Proc. Natl. Acad. Sci. USA 99: 4620-4625 [Abstract] [Full Text]  
  • Sonnleitner, E., Moll, I., Blasi, U. (2002). Functional replacement of the Escherichia coli hfq gene by the homologue of Pseudomonas aeruginosa. Microbiology 148: 883-891 [Abstract] [Full Text]  
  • Rajkumari, K., Gowrishankar, J. (2001). In Vivo Expression from the RpoS-Dependent P1 Promoter of the Osmotically Regulated proU Operon in Escherichia coli and Salmonella enterica Serovar Typhimurium: Activation by rho and hns Mutations and by Cold Stress. J. Bacteriol. 183: 6543-6550 [Abstract] [Full Text]  
  • Repoila, F., Gottesman, S. (2001). Signal Transduction Cascade for Regulation of RpoS: Temperature Regulation of DsrA. J. Bacteriol. 183: 4012-4023 [Abstract] [Full Text]  
  • Wassarman, K. M., Repoila, F., Rosenow, C., Storz, G., Gottesman, S. (2001). Identification of novel small RNAs using comparative genomics and microarrays. Genes Dev. 15: 1637-1651 [Abstract] [Full Text]  
  • GOTTESMAN, S., STORZ, G., ROSENOW, C., MAJDALANI, N., REPOILA, F., WASSARMAN, K.M. (2001). Small RNA Regulators of Translation: Mechanisms of Action and Approaches for Identifying New Small RNAs. Cold Spring Harb Symp Quant Biol 66: 353-362 [Abstract]  
  • Masse, E., Gottesman, S. (2002). A small RNA regulates the expression of genes involved in iron metabolism in Escherichiacoli. Proc. Natl. Acad. Sci. USA 99: 4620-4625 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»