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Journal of Bacteriology, March 2001, p. 2148-2150, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.2148-2150.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

DnaD Protein of Bacillus subtilis Interacts with DnaA, the Initiator Protein of Replication

Daisuke Ishigo-oka, Naotake Ogasawara, and Shigeki Moriya*

Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan

Received 25 August 2000/Accepted 14 December 2000

The yeast two-hybrid assay revealed that Bacillus subtilis DnaD, a possible component of the primosome and required for replication initiation, interacted with DnaA and DnaD itself. The mutant DnaD23 was incapable of interacting with DnaA but retained interaction with the wild-type DnaD. These results suggest that interaction between DnaD and DnaA is important for replication initiation.

* Corresponding author. Mailing address: Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5, Takayama, Ikoma, Nara 630-0101, Japan. Phone: 81-743-72-5432. Fax: 81-743-72-5439. E-mail: moriya{at}bs.aist-nara.ac.jp.

Journal of Bacteriology, March 2001, p. 2148-2150, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.2148-2150.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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