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Journal of Bacteriology, March 2001, p. 2151-2155, Vol. 183, No. 6
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.6.2151-2155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Analysis of Promoters Recognized by PvdS, an
Extracytoplasmic-Function Sigma Factor Protein from
Pseudomonas aeruginosa
Megan J.
Wilson,
Brendan J.
McMorran,
and
Iain
L.
Lamont*
Department of Biochemistry, University of
Otago, Dunedin, New Zealand
Received 21 August 2000/Accepted 30 November 2000
The alternative sigma factor PvdS is required by
Pseudomonas aeruginosa for initiation of transcription from
pyoverdine (pvd) promoters. Two divergent PvdS-dependent
promoters (pvdE and pvdF) were characterized by
deletion analysis, and the minimal promoter region for each included a
sequence element, the iron starvation (IS) box, that is present in
other pvd promoters. Site-directed mutagenesis showed that
the IS box elements were essential for promoter activity in vivo. Band
shift assays and in vitro transcription experiments showed that a
complex of PvdS and core RNA polymerase required the presence of an IS
box in order to bind to and initiate transcription from pvd
promoters. These results indicate that IS box elements participate in
sequence-specific recognition by PvdS to enable initiation of
transcription from pvd promoters and are likely to
represent a
35 sequence element for this sigma factor.
*
Corresponding author. Mailing address: Department of
Biochemistry, University of Otago, P.O. Box 56, Dunedin, New
Zealand. Phone: 64 3 479 7869. Fax: 64 3 479 7866. E-mail:
iain.lamont{at}stonebow.otago.ac.nz.

Present address: Centre for Molecular Biology and Biotechnology,
University of Queensland, Brisbane, Queensland,
Australia.
Journal of Bacteriology, March 2001, p. 2151-2155, Vol. 183, No. 6
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.6.2151-2155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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