This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wilson, M. J. Articles by Lamont, I. L. Search for Related Content PubMed PubMed Citation Articles by Wilson, M. J. Articles by Lamont, I. L.

Journal of Bacteriology, March 2001, p. 2151-2155, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.2151-2155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Analysis of Promoters Recognized by PvdS, an Extracytoplasmic-Function Sigma Factor Protein from Pseudomonas aeruginosa

Megan J. Wilson, Brendan J. McMorran, and Iain L. Lamont^*

Department of Biochemistry, University of Otago, Dunedin, New Zealand

Received 21 August 2000/Accepted 30 November 2000

The alternative sigma factor PvdS is required by Pseudomonas aeruginosa for initiation of transcription from pyoverdine (pvd) promoters. Two divergent PvdS-dependent promoters (pvdE and pvdF) were characterized by deletion analysis, and the minimal promoter region for each included a sequence element, the iron starvation (IS) box, that is present in other pvd promoters. Site-directed mutagenesis showed that the IS box elements were essential for promoter activity in vivo. Band shift assays and in vitro transcription experiments showed that a complex of PvdS and core RNA polymerase required the presence of an IS box in order to bind to and initiate transcription from pvd promoters. These results indicate that IS box elements participate in sequence-specific recognition by PvdS to enable initiation of transcription from pvd promoters and are likely to represent a 35 sequence element for this sigma factor.

---

^* Corresponding author. Mailing address: Department of Biochemistry, University of Otago, P.O. Box 56, Dunedin, New Zealand. Phone: 64 3 479 7869. Fax: 64 3 479 7866. E-mail: iain.lamont{at}stonebow.otago.ac.nz.

Present address: Centre for Molecular Biology and Biotechnology, University of Queensland, Brisbane, Queensland, Australia.

---

Journal of Bacteriology, March 2001, p. 2151-2155, Vol. 183, No. 6
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.6.2151-2155.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

This article has been cited by other articles:

• Spencer, M. R., Beare, P. A., Lamont, I. L. (2008). Role of Cell Surface Signaling in Proteolysis of an Alternative Sigma Factor in Pseudomonas aeruginosa. J. Bacteriol. 190: 4865-4869 [Abstract] [Full Text]  
• Gaines, J. M., Carty, N. L., Tiburzi, F., Davinic, M., Visca, P., Colmer-Hamood, J. A., Hamood, A. N. (2007). Regulation of the Pseudomonas aeruginosa toxA, regA and ptxR genes by the iron-starvation sigma factor PvdS under reduced levels of oxygen. Microbiology 153: 4219-4233 [Abstract] [Full Text]  
• Agnoli, K., Lowe, C. A., Farmer, K. L., Husnain, S. I., Thomas, M. S. (2006). The Ornibactin Biosynthesis and Transport Genes of Burkholderia cenocepacia Are Regulated by an Extracytoplasmic Function {sigma} Factor Which Is a Part of the Fur Regulon.. J. Bacteriol. 188: 3631-3644 [Abstract] [Full Text]  
• Lamont, I. L., Martin, L. W., Sims, T., Scott, A., Wallace, M. (2006). Characterization of a Gene Encoding an Acetylase Required for Pyoverdine Synthesis in Pseudomonas aeruginosa.. J. Bacteriol. 188: 3149-3152 [Abstract] [Full Text]  
• Wilson, M. J., Lamont, I. L. (2006). Mutational analysis of an extracytoplasmic-function sigma factor to investigate its interactions with RNA polymerase and DNA.. J. Bacteriol. 188: 1935-1942 [Abstract] [Full Text]  
• Alice, A. F., Lopez, C. S., Lowe, C. A., Ledesma, M. A., Crosa, J. H. (2006). Genetic and Transcriptional Analysis of the Siderophore Malleobactin Biosynthesis and Transport Genes in the Human Pathogen Burkholderia pseudomallei K96243. J. Bacteriol. 188: 1551-1566 [Abstract] [Full Text]  
• Maunsell, B., Adams, C., O'Gara, F. (2006). Complex regulation of AprA metalloprotease in Pseudomonas fluorescens M114: evidence for the involvement of iron, the ECF sigma factor, PbrA and pseudobactin M114 siderophore. Microbiology 152: 29-42 [Abstract] [Full Text]  
• Gaines, J. M., Carty, N. L., Colmer-Hamood, J. A., Hamood, A. N. (2005). Effect of static growth and different levels of environmental oxygen on toxA and ptxR expression in the Pseudomonas aeruginosa strain PAO1. Microbiology 151: 2263-2275 [Abstract] [Full Text]  
• Nouwens, A. S., Beatson, S. A., Whitchurch, C. B., Walsh, B. J., Schweizer, H. P., Mattick, J. S., Cordwell, S. J. (2003). Proteome analysis of extracellular proteins regulated by the las and rhl quorum sensing systems in Pseudomonas aeruginosa PAO1. Microbiology 149: 1311-1322 [Abstract] [Full Text]  
• Enz, S., Mahren, S., Menzel, C., Braun, V. (2003). Analysis of the Ferric Citrate Transport Gene Promoter of Escherichia coli. J. Bacteriol. 185: 2387-2391 [Abstract] [Full Text]  
• de Chial, M., Ghysels, B., Beatson, S. A., Geoffroy, V., Meyer, J. M., Pattery, T., Baysse, C., Chablain, P., Parsons, Y. N., Winstanley, C., Cordwell, S. J., Cornelis, P. (2003). Identification of type II and type III pyoverdine receptors from Pseudomonas aeruginosa. Microbiology 149: 821-831 [Abstract] [Full Text]  
• Lamont, I. L., Martin, L. W. (2003). Identification and characterization of novel pyoverdine synthesis genes in Pseudomonas aeruginosa. Microbiology 149: 833-842 [Abstract] [Full Text]  
• Redly, G. A., Poole, K. (2003). Pyoverdine-Mediated Regulation of FpvA Synthesis in Pseudomonas aeruginosa: Involvement of a Probable Extracytoplasmic-Function Sigma Factor, FpvI. J. Bacteriol. 185: 1261-1265 [Abstract] [Full Text]  
• Bultreys, A., Gheysen, I., Wathelet, B., Maraite, H., de Hoffmann, E. (2003). High-Performance Liquid Chromatography Analyses of Pyoverdin Siderophores Differentiate among Phytopathogenic Fluorescent Pseudomonas Species. Appl. Environ. Microbiol. 69: 1143-1153 [Abstract] [Full Text]  
• Wei, B., Huang, T., Dalwadi, H., Sutton, C. L., Bruckner, D., Braun, J. (2002). Pseudomonas fluorescens Encodes the Crohn's Disease-Associated I2 Sequence and T-Cell Superantigen. Infect. Immun. 70: 6567-6575 [Abstract] [Full Text]  
• Hunt, T. A., Peng, W.-T., Loubens, I., Storey, D. G. (2002). The Pseudomonas aeruginosa alternative sigma factor PvdS controls exotoxin A expression and is expressed in lung infections associated with cystic fibrosis. Microbiology 148: 3183-3193 [Abstract] [Full Text]  
• Ambrosi, C., Leoni, L., Visca, P. (2002). Different Responses of Pyoverdine Genes to Autoinduction in Pseudomonas aeruginosa and the Group Pseudomonas fluorescens-Pseudomonas putida. Appl. Environ. Microbiol. 68: 4122-4126 [Abstract] [Full Text]  
• Lamont, I. L., Beare, P. A., Ochsner, U., Vasil, A. I., Vasil, M. L. (2002). Siderophore-mediated signaling regulates virulence factor production in Pseudomonasaeruginosa. Proc. Natl. Acad. Sci. USA 99: 7072-7077 [Abstract] [Full Text]  
• Wilderman, P. J., Vasil, A. I., Johnson, Z., Wilson, M. J., Cunliffe, H. E., Lamont, I. L., Vasil, M. L. (2001). Characterization of an Endoprotease (PrpL) Encoded by a PvdS-Regulated Gene in Pseudomonas aeruginosa. Infect. Immun. 69: 5385-5394 [Abstract] [Full Text]