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Journal of Bacteriology, April 2001, p. 2372-2375, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2372-2375.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Conservative Duplication of Spindle Poles during Meiosis in Saccharomyces cerevisiae

Andreas Wesp,1 Susanne Prinz,2 and Gerald R. Fink1,3,*

Whitehead Institute for Biomedical Research,1 Center for Cancer Research,2 and Department of Biology,3 Massachusetts Institute of Technology, Cambridge, Massachusetts 02142

Received 21 August 2000/Accepted 11 January 2001

During sporulation in diploid Saccharomyces cerevisiae, spindle pole bodies acquire the so-called meiotic plaque, a prerequisite for spore formation. Mpc70p is a component of the meiotic plaque and is thus essential for spore formation. We show here that MPC70/mpc70 heterozygous strains most often produce two spores instead of four and that these spores are always nonsisters. In wild-type strains, Mpc70p localizes to all four spindle pole bodies, whereas in MPC70/mpc70 strains Mpc70p localizes to only two of the four spindle pole bodies, and these are always nonsisters. Our data can be explained by conservative spindle pole body distribution in which the two newly synthesized meiosis II spindle pole bodies of MPC70/mpc70 strains lack Mpc70p.

* Corresponding author. Mailing address: Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, MA 02142-1479. Phone: (617) 258-5215. Fax: (617) 258-9872. E-mail: fink{at}wi.mit.edu.

Journal of Bacteriology, April 2001, p. 2372-2375, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2372-2375.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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