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Journal of Bacteriology, April 2001, p. 2376-2379, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2376-2379.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Establishing Lysogenic Transcription in the Temperate Coliphage 186

Petra J. Neufing,dagger Keith E. Shearwin, and J. Barry Egan*

Department of Molecular Biosciences, Adelaide University, South Australia 5005, Australia

Received 8 June 2000/Accepted 10 January 2001

A single-copy chromosomal reporter system was used to measure the intrinsic strengths and interactions between the three promoters involved in the establishment of lysogeny by coliphage 186. The maintenance lysogenic promoter pL for the immunity repressor gene cI is intrinsically ~20-fold weaker than the lytic promoter pR. These promoters are arranged face-to-face, and transcription from pL is further weakened some 14-fold by the activity of pR. Efficient establishment of lysogeny requires the pE promoter, which lies upstream of pL and is activated by the phage CII protein to a level comparable to that of pR. Transcription of pE is less sensitive to converging pR transcription and raises cI transcription at least 55-fold. The pE promoter does not occlude pL but inhibits lytic transcription by 50%. This interference is not due to bound CII preventing elongation of the lytic transcript. The pE RNA is antisense to the anti-immune repressor gene apl, but any role of this in the establishment of lysogeny appears to be minimal.

* Corresponding author. Mailing address: Department of Molecular Biosciences, Adelaide University, South Australia 5005, Australia. Phone: 61 8 8303 5361. Fax: 61 8 8303 4348. E-mail: barry.egan{at}adelaide.edu.au.

dagger Present address: School of Medicine, Flinders Medical Centre, Flinders University, Bedford Park, South Australia 5042, Australia.

Journal of Bacteriology, April 2001, p. 2376-2379, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2376-2379.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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