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Journal of Bacteriology, April 2001, p. 2380-2383, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2380-2383.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

FosB, a Cysteine-Dependent Fosfomycin Resistance Protein under the Control of sigma W, an Extracytoplasmic-Function sigma  Factor in Bacillus subtilis

Min Cao,1 Bryan A. Bernat,2 Zhepeng Wang,2 Richard N. Armstrong,2 and John D. Helmann1,*

Department of Microbiology, Cornell University, Ithaca, New York 14853-8101,1 and Department of Biochemistry and the Center in Molecular Toxicology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-01462

Received 5 October 2000/Accepted 27 December 2000

We demonstrate that the Bacillus subtilis fosB(yndN) gene encodes a fosfomycin resistance protein. Expression of fosB requires sigma W, and both fosB and sigW mutants are fosfomycin sensitive. FosB is a metallothiol transferase related to the FosA class of Mn2+-dependent glutathione transferases but with a preference for Mg2+ and L-cysteine as cofactors.

* Corresponding author. Mailing address: Department of Microbiology, Cornell University, Ithaca, NY 14853-8101. Phone: (607) 255-6570. Fax: (607) 255-3904. E-mail: jdh9{at}cornell.edu.

Journal of Bacteriology, April 2001, p. 2380-2383, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2380-2383.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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