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Journal of Bacteriology, April 2001, p. 2389-2393, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2389-2393.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Transcriptional Activation of the Bacillus subtilis ackA Promoter Requires Sequences Upstream of the CcpA Binding Site

Tessa R. Moir-Blais, Frank J. Grundy, and Tina M. Henkin*

Department of Microbiology, The Ohio State University, Columbus, Ohio 43210

Received 4 October 2000/Accepted 8 January 2001

Carbon catabolite protein A (CcpA) is a global regulator of carbon metabolism in gram-positive bacteria, repressing transcription of genes for the utilization of secondary carbon sources in the presence of a readily metabolized carbon source and activating transcription of genes, such as ackA and pta, that are required for carbon excretion. The promoter region of the Bacillus subtilis ackA gene contains two catabolite responsive elements (cre sites), of which only the site closest to the promoter (cre2) binds CcpA to activate transcription. A region immediately upstream of the cre2 site is also important for transcriptional activation. The required elements in this region were further defined by mutagenesis. CcpA binds to the ackA promoter region in gel shift assays even in the presence of mutations in the upstream element that block transcriptional activation, indicating that this region has a function other than promoting binding of CcpA.

* Corresponding author. Mailing address: Department of Microbiology, The Ohio State University, 484 West 12th Ave., Columbus, Ohio 43210. Phone: (614) 688-3831. Fax: (614) 292-8120. E-mail: henkin.3{at}osu.edu.

Journal of Bacteriology, April 2001, p. 2389-2393, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2389-2393.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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