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Journal of Bacteriology, April 2001, p. 2389-2393, Vol. 183, No. 7
Department of Microbiology, The Ohio State
University, Columbus, Ohio 43210
Received 4 October 2000/Accepted 8 January 2001
Carbon catabolite protein A (CcpA) is a global regulator of carbon
metabolism in gram-positive bacteria, repressing transcription of genes
for the utilization of secondary carbon sources in the presence of a
readily metabolized carbon source and activating transcription of
genes, such as ackA and pta, that are required for carbon excretion. The promoter region of the Bacillus
subtilis ackA gene contains two catabolite responsive elements
(cre sites), of which only the site closest to the promoter
(cre2) binds CcpA to activate transcription. A region
immediately upstream of the cre2 site is also important for
transcriptional activation. The required elements in this region were
further defined by mutagenesis. CcpA binds to the ackA
promoter region in gel shift assays even in the presence of mutations
in the upstream element that block transcriptional activation,
indicating that this region has a function other than promoting binding
of CcpA.
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.7.2389-2393.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Transcriptional Activation of the Bacillus
subtilis ackA Promoter Requires Sequences Upstream of the CcpA
Binding Site
*
Corresponding author. Mailing address: Department of
Microbiology, The Ohio State University, 484 West 12th Ave., Columbus, Ohio 43210. Phone: (614) 688-3831. Fax: (614) 292-8120. E-mail: henkin.3{at}osu.edu.
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