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Journal of Bacteriology, April 2001, p. 2394-2397, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2394-2397.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

New Insight into the Role of the PhaP Phasin of Ralstonia eutropha in Promoting Synthesis of Polyhydroxybutyrate

Gregory M. York,1 JoAnne Stubbe,1,2 and Anthony J. Sinskey1,*

Department of Biology1 and Department of Chemistry,2 Massachusetts Institute of Technology, Cambridge, Massachusetts 02139

Received 20 October 2000/Accepted 17 January 2001

Phasins are proteins that are proposed to play important roles in polyhydroxyalkanoate synthesis and granule formation. Here the phasin PhaP of Ralstonia eutropha has been analyzed with regard to its role in the synthesis of polyhydroxybutyrate (PHB). Purified recombinant PhaP, antibodies against PhaP, and an R. eutropha phaP deletion strain have been generated for this analysis. Studies with the phaP deletion strain show that PhaP must accumulate to high levels in order to play its normal role in PHB synthesis and that the accumulation of PhaP to low levels is functionally equivalent to the absence of PhaP. PhaP positively affects PHB synthesis under growth conditions which promote production of PHB to low, intermediate, or high levels. The levels of PhaP generally parallel levels of PHB in cells. The results are consistent with models whereby PhaP promotes PHB synthesis by regulating the surface/volume ratio of PHB granules or by interacting with polyhydroxyalkanoate synthase and indicate that PhaP plays an important role in PHB synthesis from the early stages in PHB production and across a range of growth conditions.

* Corresponding author. Mailing address: Bldg. 68-370, Department of Biology, Massachusetts Institute of Technology, 77 Massachusetts Ave., Cambridge, MA 02139. Phone: (617) 253-6721. Fax: (617) 253-8550. E-mail: asinskey{at}mit.edu.

Journal of Bacteriology, April 2001, p. 2394-2397, Vol. 183, No. 7
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.7.2394-2397.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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