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Journal of Bacteriology, April 2001, p. 2411-2416, Vol. 183, No. 8
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.8.2411-2416.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
GcpE Is Involved in the
2-C-Methyl-D-Erythritol 4-Phosphate Pathway of
Isoprenoid Biosynthesis in Escherichia coli
Boran
Altincicek,*
Ann-Kristin
Kollas,
Silke
Sanderbrand,
Jochen
Wiesner,
Martin
Hintz,
Ewald
Beck, and
Hassan
Jomaa
Institute of Biochemistry, Academic Hospital
Centre, Justus Liebig University, D-35392 Giessen, Germany
Received 17 November 2000/Accepted 16 January 2001
In a variety of organisms, including plants and several eubacteria,
isoprenoids are synthesized by the mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP)
pathway. Although different enzymes of this pathway have been
described, the terminal biosynthetic steps of the MEP pathway have not
been fully elucidated. In this work, we demonstrate that the
gcpE gene of Escherichia coli is involved in
this pathway. E. coli cells were genetically
engineered to utilize exogenously provided mevalonate for isoprenoid
biosynthesis by the mevalonate pathway. These cells were then deleted
for the essential gcpE gene and were viable only if the
medium was supplemented with mevalonate or the cells were complemented
with an episomal copy of gcpE.
*
Corresponding author. Mailing address: Institute of
Biochemistry, Academic Hospital Centre, Justus Liebig University,
Friedrichstr. 24, D-35392 Giessen, Germany. Phone: 49-6419947528. Fax: 49-6419947529. E-mail:
Boran.Altincicek{at}biochemie.med.uni-giessen.de.
Journal of Bacteriology, April 2001, p. 2411-2416, Vol. 183, No. 8
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.8.2411-2416.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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