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Journal of Bacteriology, April 2001, p. 2411-2416, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2411-2416.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

GcpE Is Involved in the 2-C-Methyl-D-Erythritol 4-Phosphate Pathway of Isoprenoid Biosynthesis in Escherichia coli

Boran Altincicek,* Ann-Kristin Kollas, Silke Sanderbrand, Jochen Wiesner, Martin Hintz, Ewald Beck, and Hassan Jomaa

Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, D-35392 Giessen, Germany

Received 17 November 2000/Accepted 16 January 2001

In a variety of organisms, including plants and several eubacteria, isoprenoids are synthesized by the mevalonate-independent 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway. Although different enzymes of this pathway have been described, the terminal biosynthetic steps of the MEP pathway have not been fully elucidated. In this work, we demonstrate that the gcpE gene of Escherichia coli is involved in this pathway. E. coli cells were genetically engineered to utilize exogenously provided mevalonate for isoprenoid biosynthesis by the mevalonate pathway. These cells were then deleted for the essential gcpE gene and were viable only if the medium was supplemented with mevalonate or the cells were complemented with an episomal copy of gcpE.

* Corresponding author. Mailing address: Institute of Biochemistry, Academic Hospital Centre, Justus Liebig University, Friedrichstr. 24, D-35392 Giessen, Germany. Phone: 49-6419947528. Fax: 49-6419947529. E-mail: Boran.Altincicek{at}biochemie.med.uni-giessen.de.

Journal of Bacteriology, April 2001, p. 2411-2416, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2411-2416.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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