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Journal of Bacteriology, April 2001, p. 2535-2542, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2535-2542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Efficiency of Recombination Reactions Catalyzed by Class 1 Integron Integrase IntI1

Christina M. Collis,1 Gavin D. Recchia,1,2 Mi-Jurng Kim,2 H. W. Stokes,2 and Ruth M. Hall1,*

CSIRO Molecular Science, Sydney Laboratory, North Ryde, New South Wales 1670,1 and School of Biological Sciences, Macquarie University Sydney, New South Wales 2109,2 Australia

Received 11 August 2000/Accepted 16 January 2001

The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between attI1 and a 59-be, or between two attI1 sites, were compared. Recombination events involving two attI1 sites were significantly less efficient than the reactions in which a 59-be participated, and the attI1 × 59-be reaction was generally preferred over the 59-be × 59-be reaction. Recombination of attI1 with secondary sites was less efficient than the 59-be × secondary site reaction.

* Corresponding author. Mailing address: CSIRO Molecular Science, Sydney Laboratory, P.O. Box 184, North Ryde, NSW 1670, Australia. Phone: (612) 9490-5162. Fax: (612) 9490-5005. E-mail: ruth.hall{at}molsci.csiro.au.

Journal of Bacteriology, April 2001, p. 2535-2542, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2535-2542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.