This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Collis, C. M.
Right arrow Articles by Hall, R. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Collis, C. M.
Right arrow Articles by Hall, R. M.

 Previous Article  |  Next Article 

Journal of Bacteriology, April 2001, p. 2535-2542, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2535-2542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Efficiency of Recombination Reactions Catalyzed by Class 1 Integron Integrase IntI1

Christina M. Collis,1 Gavin D. Recchia,1,2 Mi-Jurng Kim,2 H. W. Stokes,2 and Ruth M. Hall1,*

CSIRO Molecular Science, Sydney Laboratory, North Ryde, New South Wales 1670,1 and School of Biological Sciences, Macquarie University Sydney, New South Wales 2109,2 Australia

Received 11 August 2000/Accepted 16 January 2001

The class 1 integron integrase, IntI1, recognizes two distinct types of recombination sites, attI sites, found in integrons, and members of the 59-be family, found in gene cassettes. The efficiencies of the integrative version of the three possible reactions, i.e., between two 59-be, between attI1 and a 59-be, or between two attI1 sites, were compared. Recombination events involving two attI1 sites were significantly less efficient than the reactions in which a 59-be participated, and the attI1 × 59-be reaction was generally preferred over the 59-be × 59-be reaction. Recombination of attI1 with secondary sites was less efficient than the 59-be × secondary site reaction.

* Corresponding author. Mailing address: CSIRO Molecular Science, Sydney Laboratory, P.O. Box 184, North Ryde, NSW 1670, Australia. Phone: (612) 9490-5162. Fax: (612) 9490-5005. E-mail: ruth.hall{at}molsci.csiro.au.

Journal of Bacteriology, April 2001, p. 2535-2542, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2535-2542.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Rowe-Magnus, D. A. (2009). Integrase-directed recovery of functional genes from genomic libraries. Nucleic Acids Res 37: e118-e118 [Abstract] [Full Text]  
  • Rodriguez-Minguela, C. M., Apajalahti, J. H. A., Chai, B., Cole, J. R., Tiedje, J. M. (2009). Worldwide Prevalence of Class 2 Integrases outside the Clinical Setting Is Associated with Human Impact. Appl. Environ. Microbiol. 75: 5100-5110 [Abstract] [Full Text]  
  • Huang, L., Cagnon, C., Caumette, P., Duran, R. (2009). First Gene Cassettes of Integrons as Targets in Finding Adaptive Genes in Metagenomes. Appl. Environ. Microbiol. 75: 3823-3825 [Abstract] [Full Text]  
  • Marquez, C., Labbate, M., Ingold, A. J., Chowdhury, P. R., Ramirez, M. S., Centron, D., Borthagaray, G., Stokes, H. W. (2008). Recovery of a Functional Class 2 Integron from an Escherichia coli Strain Mediating a Urinary Tract Infection. Antimicrob. Agents Chemother. 52: 4153-4154 [Abstract] [Full Text]  
  • Demarre, G., Frumerie, C., Gopaul, D. N., Mazel, D. (2007). Identification of key structural determinants of the IntI1 integron integrase that influence attC x attI1 recombination efficiency. Nucleic Acids Res 35: 6475-6489 [Abstract] [Full Text]  
  • Coleman, N. V., Holmes, A. J. (2005). The native Pseudomonas stutzeri strain Q chromosomal integron can capture and express cassette-associated genes. Microbiology 151: 1853-1864 [Abstract] [Full Text]  
  • Gillings, M. R., Holley, M. P., Stokes, H. W., Holmes, A. J. (2005). Integrons in Xanthomonas: A source of species genome diversity. Proc. Natl. Acad. Sci. USA 102: 4419-4424 [Abstract] [Full Text]  
  • Biskri, L., Bouvier, M., Guerout, A.-M., Boisnard, S., Mazel, D. (2005). Comparative Study of Class 1 Integron and Vibrio cholerae Superintegron Integrase Activities. J. Bacteriol. 187: 1740-1750 [Abstract] [Full Text]  
  • Quinteira, S., Sousa, J. C., Peixe, L. (2005). Characterization of In100, a New Integron Carrying a Metallo-{beta}-Lactamase and a Carbenicillinase, from Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 49: 451-453 [Abstract] [Full Text]  
  • Collis, C. M., Hall, R. M. (2004). Comparison of the structure-activity relationships of the integron-associated recombination sites attI3 and attI1 reveals common features. Microbiology 150: 1591-1601 [Abstract] [Full Text]  
  • Biskri, L., Mazel, D. (2003). Erythromycin Esterase Gene ere(A) Is Located in a Functional Gene Cassette in an Unusual Class 2 Integron. Antimicrob. Agents Chemother. 47: 3326-3331 [Abstract] [Full Text]  
  • Rowe-Magnus, D. A., Guerout, A.-M., Biskri, L., Bouige, P., Mazel, D. (2003). Comparative Analysis of Superintegrons: Engineering Extensive Genetic Diversity in the Vibrionaceae. Genome Res 13: 428-442 [Abstract] [Full Text]  
  • Holmes, A. J., Holley, M. P., Mahon, A., Nield, B., Gillings, M., Stokes, H. W. (2003). Recombination Activity of a Distinctive Integron-Gene Cassette System Associated with Pseudomonas stutzeri Populations in Soil. J. Bacteriol. 185: 918-928 [Abstract] [Full Text]  
  • Collis, C. M., Kim, M.-J., Partridge, S. R., Stokes, H. W., Hall, R. M. (2002). Characterization of the Class 3 Integron and the Site-Specific Recombination System It Determines. J. Bacteriol. 184: 3017-3026 [Abstract] [Full Text]  
  • Stokes, H. W., Holmes, A. J., Nield, B. S., Holley, M. P., Nevalainen, K. M. H., Mabbutt, B. C., Gillings, M. R. (2001). Gene Cassette PCR: Sequence-Independent Recovery of Entire Genes from Environmental DNA. Appl. Environ. Microbiol. 67: 5240-5246 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»