Allelic Diversity and Recombination in Campylobacter jejuni

doi:10.1128/JB.183.8.2553-2559.2001

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Journal of Bacteriology, April 2001, p. 2553-2559, Vol. 183, No. 8
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.8.2553-2559.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Allelic Diversity and Recombination in Campylobacter jejuni

Sebastian Suerbaum,¹^,^* Marc Lohrengel,¹ Agnes Sonnevend,¹ Florian Ruberg,² and Manfred Kist²

Institut für Hygiene und Mikrobiologie der Universität Würzburg, D-97080 Würzburg,¹ and Institut für Medizinische Mikrobiologie und Hygiene der Universität Freiburg, D-79104 Freiburg,² Germany

Received 12 September 2000/Accepted 29 January 2001

The allelic diversity and population structure of Campylobacter jejuni were studied by multilocus nucleotide sequence analysis.Sequences from seven housekeeping genes were obtained from 32C. jejuni isolates isolated from enteritis patients in Germany,Hungary, Thailand, and the United States. Also included was strainNCTC 11168, the complete genomic sequence of which has recentlybeen published. For all loci analyzed, multiple strains carriedidentical alleles. The frequency of synonymous and nonsynonymoussequence polymorphisms was low. The number of unique alleles perlocus ranged from 9 to 15. These alleles occurred in 31 differentcombinations (sequence types), so that all but two pairs of strainscould be distinguished from each other. Sequences were analyzedfor evidence of recombination by the homoplasy test and splitdecomposition. These analyses showed that intraspecific recombinationis frequent in C. jejuni and has generated extensive diversityof allelic profiles from a small number of polymorphicnucleotides.

^* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Josef-Schneider-Stra $beta$ e2, D-97080 Würzburg, Germany. Phone: 49 931 201 3949. Fax: 49931 201 3445. E-mail: ssuerbaum{at}hygiene.uni-wuerzburg.de.

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