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Journal of Bacteriology, April 2001, p. 2553-2559, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2553-2559.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Allelic Diversity and Recombination in Campylobacter jejuni

Sebastian Suerbaum,1,* Marc Lohrengel,1 Agnes Sonnevend,1 Florian Ruberg,2 and Manfred Kist2

Institut für Hygiene und Mikrobiologie der Universität Würzburg, D-97080 Würzburg,1 and Institut für Medizinische Mikrobiologie und Hygiene der Universität Freiburg, D-79104 Freiburg,2 Germany

Received 12 September 2000/Accepted 29 January 2001

The allelic diversity and population structure of Campylobacter jejuni were studied by multilocus nucleotide sequence analysis. Sequences from seven housekeeping genes were obtained from 32 C. jejuni isolates isolated from enteritis patients in Germany, Hungary, Thailand, and the United States. Also included was strain NCTC 11168, the complete genomic sequence of which has recently been published. For all loci analyzed, multiple strains carried identical alleles. The frequency of synonymous and nonsynonymous sequence polymorphisms was low. The number of unique alleles per locus ranged from 9 to 15. These alleles occurred in 31 different combinations (sequence types), so that all but two pairs of strains could be distinguished from each other. Sequences were analyzed for evidence of recombination by the homoplasy test and split decomposition. These analyses showed that intraspecific recombination is frequent in C. jejuni and has generated extensive diversity of allelic profiles from a small number of polymorphic nucleotides.

* Corresponding author. Mailing address: Institut für Hygiene und Mikrobiologie der Universität Würzburg, Josef-Schneider-Strabeta e 2, D-97080 Würzburg, Germany. Phone: 49 931 201 3949. Fax: 49 931 201 3445. E-mail: ssuerbaum{at}hygiene.uni-wuerzburg.de.

Journal of Bacteriology, April 2001, p. 2553-2559, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2553-2559.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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