Previous Article | Next Article 
Journal of Bacteriology, April 2001, p. 2560-2569, Vol. 183, No. 8
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.8.2560-2569.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Mutually Exclusive Distribution of
IS1548 and GBSi1, an Active Group II Intron Identified in
Human Isolates of Group B Streptococci
Margareta
Granlund,1,*
François
Michel,2 and
Mari
Norgren1
Department of Clinical Bacteriology, Umeå
University, S-901 85 Umeå, Sweden,1 and Centre
de Génétique Moléculaire du CNRS, 91190 Gif-sur-Yvette, France2
Received 29 August 2000/Accepted 24 January 2001
The present study shows that active, self-splicing group II intron
GBSi1 is located downstream of the C5a-peptidase gene, scpB, in some group B streptococcus (GBS) isolates that
lack insertion sequence IS1548. IS1548 was
previously reported to be often present at the scpB locus
in GBS isolated in association with endocarditis. Since none of 67 GBS
isolates examined, 40 of which were of serotype III, harbored both
IS1548 and GBSi1, these two elements are suggested to be
markers for different genetic lineages in GBS serotype III. The DNA
region downstream of scpB in GBS isolates harboring either GBSi1, IS1548, or none of these mobile elements was found to encode the
laminin binding protein, Lmb, which shows sequence similarities to a
family of streptococcal adhesins. IS1548 is inserted 9 bp upstream of the putative promoter for lmb, while the
insertion site for GBSi1 is located 88 bp further upstream. Sequences
highly similar to GBSi1 exist also in Streptococcus
pneumoniae. An inverted repeat sequence, with features typical of
transcription terminators, was identified immediately upstream of the
insertion site for the group II intron both in the GBS and S. pneumoniae sequences. This motif is suggested to constitute a
target for the GBS intron as well as for rather closely related introns
in Bacillus halodurans, Pseudomonas alcaligenes, and
Pseudomonas putida. When transcripts containing the GBSi1
intron were incubated at high concentrations of ammonium and magnesium,
a major product with the expected length and sequence for the ligated
exons was generated. Unlike, however, all members of group II
investigated so far, the excised intron was in linear, rather than in a
branched (lariat), form.
*
Corresponding author. Mailing address: Department of
Clinical Bacteriology, Umeå University, S-901 85 Umeå, Sweden. Phone: 46-90-7851772. Fax: 46-90-7852225. E-mail:
Margareta.Granlund{at}climi.umu.se.
Journal of Bacteriology, April 2001, p. 2560-2569, Vol. 183, No. 8
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.8.2560-2569.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Leon, G., Roy, P. H.
(2009). Group IIC intron mobility into attC sites involves a bulged DNA stem-loop motif. RNA
15: 1543-1553
[Abstract]
[Full Text]
-
Domelier, A.-S., van der Mee-Marquet, N., Sizaret, P.-Y., Hery-Arnaud, G., Lartigue, M.-F., Mereghetti, L., Quentin, R.
(2009). Molecular Characterization and Lytic Activities of Streptococcus agalactiae Bacteriophages and Determination of Lysogenic-Strain Features. J. Bacteriol.
191: 4776-4785
[Abstract]
[Full Text]
-
Zhuang, F., Karberg, M., Perutka, J., Lambowitz, A. M.
(2009). EcI5, a group IIB intron with high retrohoming frequency: DNA target site recognition and use in gene targeting. RNA
15: 432-449
[Abstract]
[Full Text]
-
Roitzsch, M., Pyle, A. M.
(2009). The linear form of a group II intron catalyzes efficient autocatalytic reverse splicing, establishing a potential for mobility. RNA
15: 473-482
[Abstract]
[Full Text]
-
van der Mee-Marquet, N., Jouannet, C., Domelier, A.-S., Arnault, L., Lartigue, M.-F., Quentin, R.
(2009). Genetic diversity of Streptococcus agalactiae strains and density of vaginal carriage. J Med Microbiol
58: 169-173
[Abstract]
[Full Text]
-
Domelier, A.-S., van der Mee-Marquet, N., Arnault, L., Mereghetti, L., Lanotte, P., Rosenau, A., Lartigue, M.-F., Quentin, R.
(2008). Molecular characterization of erythromycin-resistant Streptococcus agalactiae strains. J Antimicrob Chemother
62: 1227-1233
[Abstract]
[Full Text]
-
Simon, D. M., Clarke, N. A.C., McNeil, B. A., Johnson, I., Pantuso, D., Dai, L., Chai, D., Zimmerly, S.
(2008). Group II introns in Eubacteria and Archaea: ORF-less introns and new varieties. RNA
14: 1704-1713
[Abstract]
[Full Text]
-
Quiroga, C., Roy, P. H., Centron, D.
(2008). The S.ma.I2 class C group II intron inserts at integron attC sites. Microbiology
154: 1341-1353
[Abstract]
[Full Text]
-
Toor, N., Keating, K. S., Taylor, S. D., Pyle, A. M.
(2008). Crystal Structure of a Self-Spliced Group II Intron. Science
320: 77-82
[Abstract]
[Full Text]
-
Hery-Arnaud, G., Bruant, G., Lanotte, P., Brun, S., Picard, B., Rosenau, A., van der Mee-Marquet, N., Rainard, P., Quentin, R., Mereghetti, L.
(2007). Mobile Genetic Elements Provide Evidence for a Bovine Origin of Clonal Complex 17 of Streptococcus agalactiae. Appl. Environ. Microbiol.
73: 4668-4672
[Abstract]
[Full Text]
-
Robart, A. R., Seo, W., Zimmerly, S.
(2007). Insertion of group II intron retroelements after intrinsic transcriptional terminators. Proc. Natl. Acad. Sci. USA
104: 6620-6625
[Abstract]
[Full Text]
-
Toor, N., Robart, A. R., Christianson, J., Zimmerly, S.
(2006). Self-splicing of a group IIC intron: 5' exon recognition and alternative 5' splicing events implicate the stem-loop motif of a transcriptional terminator. Nucleic Acids Res
34: 6461-6471
[Abstract]
[Full Text]
-
Domelier, A.-S., van der Mee-Marquet, N., Grandet, A., Mereghetti, L., Rosenau, A., Quentin, R.
(2006). Loss of Catabolic Function in Streptococcus agalactiae Strains and Its Association with Neonatal Meningitis.. J. Clin. Microbiol.
44: 3245-3250
[Abstract]
[Full Text]
-
Nagano, N., Nagano, Y., Nakano, R., Okamoto, R., Inoue, M.
(2006). Genetic diversity of the C protein beta-antigen gene and its upstream regions within clonally related groups of type Ia and Ib group B streptococci.. Microbiology
152: 771-778
[Abstract]
[Full Text]
-
COSTA, M., MICHEL, F., TORO, N.
(2006). Potential for alternative intron-exon pairings in group II intron RmInt1 from Sinorhizobium meliloti and its relatives. RNA
12: 338-341
[Abstract]
[Full Text]
-
Li-Pook-Than, J., Bonen, L.
(2006). Multiple physical forms of excised group II intron RNAs in wheat mitochondria.. Nucleic Acids Res
34: 2782-2790
[Abstract]
[Full Text]
-
Hery-Arnaud, G., Bruant, G., Lanotte, P., Brun, S., Rosenau, A., van der Mee-Marquet, N., Quentin, R., Mereghetti, L.
(2005). Acquisition of Insertion Sequences and the GBSi1 Intron by Streptococcus agalactiae Isolates Correlates with the Evolution of the Species. J. Bacteriol.
187: 6248-6252
[Abstract]
[Full Text]
-
Luan, S.-L., Granlund, M., Sellin, M., Lagergard, T., Spratt, B. G., Norgren, M.
(2005). Multilocus Sequence Typing of Swedish Invasive Group B Streptococcus Isolates Indicates a Neonatally Associated Genetic Lineage and Capsule Switching. J. Clin. Microbiol.
43: 3727-3733
[Abstract]
[Full Text]
-
Fernandez-Lopez, M., Munoz-Adelantado, E., Gillis, M., Willems, A., Toro, N.
(2005). Dispersal and Evolution of the Sinorhizobium meliloti Group II RmInt1 Intron in Bacteria that Interact with Plants. Mol Biol Evol
22: 1518-1528
[Abstract]
[Full Text]
-
Lindahl, G., Stalhammar-Carlemalm, M., Areschoug, T.
(2005). Surface Proteins of Streptococcus agalactiae and Related Proteins in Other Bacterial Pathogens. Clin. Microbiol. Rev.
18: 102-127
[Abstract]
[Full Text]
-
ROBART, A. R., MONTGOMERY, N. K., SMITH, K. L., ZIMMERLY, S.
(2004). Principles of 3' splice site selection and alternative splicing for an unusual group II intron from Bacillus anthracis. RNA
10: 854-862
[Abstract]
[Full Text]
-
Dmitriev, A., Shen, A., Shen, X., Yang, Y.
(2004). ISSa4-Based Differentiation of Streptococcus agalactiae Strains and Identification of Multiple Target Sites for ISSa4 Insertions. J. Bacteriol.
186: 1106-1109
[Abstract]
[Full Text]
-
Kong, F., Martin, D., James, G., Gilbert, G. L.
(2003). Towards a genotyping system for Streptococcus agalactiae (group B streptococcus): use of mobile genetic elements in Australasian invasive isolates. J Med Microbiol
52: 337-344
[Abstract]
[Full Text]
-
Podar, M., Mullineaux, L., Huang, H.-R., Perlman, P. S., Sogin, M. L.
(2002). Bacterial Group II Introns in a Deep-Sea Hydrothermal Vent Environment. Appl. Environ. Microbiol.
68: 6392-6398
[Abstract]
[Full Text]
-
Tettelin, H., Masignani, V., Cieslewicz, M. J., Eisen, J. A., Peterson, S., Wessels, M. R., Paulsen, I. T., Nelson, K. E., Margarit, I., Read, T. D., Madoff, L. C., Wolf, A. M., Beanan, M. J., Brinkac, L. M., Daugherty, S. C., DeBoy, R. T., Durkin, A. S., Kolonay, J. F., Madupu, R., Lewis, M. R., Radune, D., Fedorova, N. B., Scanlan, D., Khouri, H., Mulligan, S., Carty, H. A., Cline, R. T., Van Aken, S. E., Gill, J., Scarselli, M., Mora, M., Iacobini, E. T., Brettoni, C., Galli, G., Mariani, M., Vegni, F., Maione, D., Rinaudo, D., Rappuoli, R., Telford, J. L., Kasper, D. L., Grandi, G., Fraser, C. M.
(2002). Complete genome sequence and comparative genomic analysis of an emerging human pathogen, serotype V Streptococcus agalactiae. Proc. Natl. Acad. Sci. USA
99: 12391-12396
[Abstract]
[Full Text]
-
Dai, L., Zimmerly, S.
(2002). Compilation and analysis of group II intron insertions in bacterial genomes: evidence for retroelement behavior. Nucleic Acids Res
30: 1091-1102
[Abstract]
[Full Text]
-
Bohnsack, J. F., Whiting, A. A., Bradford, R. D., Van Frank, B. K., Takahashi, S., Adderson, E. E.
(2002). Long-Range Mapping of the Streptococcus agalactiae Phylogenetic Lineage Restriction Digest Pattern Type III-3 Reveals Clustering of Virulence Genes. Infect. Immun.
70: 134-139
[Abstract]
[Full Text]