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Journal of Bacteriology, April 2001, p. 2677-2681, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2677-2681.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Characterization of Brucella suis clpB and clpAB Mutants and Participation of the Genes in Stress Responses

Euloge Ekaza, Jacques Teyssier, Safia Ouahrani-Bettache, Jean-Pierre Liautard, and Stephan Köhler*

Institut National de la Santé et de la Recherche Médicale U-431, Université Montpellier II, F-34095 Montpellier, France

Received 23 October 2000/Accepted 26 January 2001

Pathogens often encounter stressful conditions inside their hosts. In the attempt to characterize the stress response in Brucella suis, a gene highly homologous to Escherichia coli clpB was isolated from Brucella suis, and the deduced amino acid sequence showed features typical of the ClpB ATPase family of stress response proteins. Under high-temperature stress conditions, ClpB of B. suis was induced, and an isogenic B. suis clpB mutant showed increased sensitivity to high temperature, but also to ethanol stress and acid pH. The effects were reversible by complementation. Simultaneous inactivation of clpA and clpB resulted in a mutant that was sensitive to oxidative stress. In B. suis expressing gfp, ClpA but not ClpB participated in degradation of the green fluorescent protein at 42°C. We concluded that ClpB was responsible for tolerance to several stresses and that the lethality caused by harsh environmental conditions may have similar molecular origins.

* Corresponding author. Mailing address: INSERM U-431, Université Montpellier II, C.P. 100, Pl. E. Bataillon, 34095 Montpellier, France. Phone: (33) 4 67 14 42 38. Fax: (33) 4 67 14 33 38. E-mail: kohler{at}crit.univ-montp2.fr.

Journal of Bacteriology, April 2001, p. 2677-2681, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2677-2681.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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