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Journal of Bacteriology, April 2001, p. 2696-2699, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2696-2699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Development of a New Integration Site within the Bacillus subtilis Chromosome and Construction of Compatible Expression Cassettes

Barbara Härtl, Wolfgang Wehrl, Thomas Wiegert, Georg Homuth, and Wolfgang Schumann*

Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany

Received 16 October 2000/Accepted 8 January 2001

The Bacillus subtilis lacA gene, coding for beta -galactosidase, has been explored as a new site able to accept DNA sequences from nonreplicating delivery vectors. Two such delivery expression vectors have been constructed and shown to be useful in obtaining regulated expression from the chromosomal location. In another experiment, it was shown that the integration of a regulatory gene at the lacA locus was able to control the expression of a transcriptional fusion at the amyE locus. These experiments demonstrate that both integration sites can be used simultaneously to obtain regulated expression of desired genes.

* Corresponding author. Mailing address: Institute of Genetics, University of Bayreuth, D-95440 Bayreuth, Germany. Phone: 49 (921) 552708. Fax: 49 (921) 552710. E-mail: wolfgang.schumann{at}uni-bayreuth.de.

Journal of Bacteriology, April 2001, p. 2696-2699, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2696-2699.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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