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 Previous Article

Journal of Bacteriology, April 2001, p. 2709-2714, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2709-2714.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Growth Inhibition Caused by Overexpression of the Structural Gene for Glutamate Dehydrogenase (gdhA) from Klebsiella aerogenes

Brian K. Janes, Pablo J. Pomposiello,dagger Ana Perez-Matos, David J. Najarian, Thomas J. Goss, and Robert A. Bender*

Department of Biology, The University of Michigan, Ann Arbor, Michigan 48109-1048

Received 23 October 2000/Accepted 1 February 2001

Two linked mutations affecting glutamate dehydrogenase (GDH) formation (gdh-1 and rev-2) had been isolated at a locus near the trp cluster in Klebsiella aerogenes. The properties of these two mutations were consistent with those of a locus containing either a regulatory gene or a structural gene. The gdhA gene from K. aerogenes was cloned and sequenced, and an insertion mutation was generated and shown to be linked to trp. A region of gdhA from a strain bearing gdh-1 was sequenced and shown to have a single-base-pair change, confirming that the locus defined by gdh-1 is the structural gene for GDH. Mutants with the same phenotype as rev-2 were isolated, and their sequences showed that the mutations were located in the promoter region of the gdhA gene. The linkage of gdhA to trp in K. aerogenes was explained by postulating an inversion of the genetic map relative to other enteric bacteria. Strains that bore high-copy-number clones of gdhA displayed an auxotrophy that was interpreted as a limitation for alpha -ketoglutarate and consequently for succinyl-coenzyme A (CoA). Three lines of evidence supported this interpretation: high-copy-number clones of the enzymatically inactive gdhA1 allele showed no auxotrophy, repression of GDH expression by the nitrogen assimilation control protein (NAC) relieved the auxotrophy, and addition of compounds that could increase the alpha -ketoglutarate supply or reduce the succinyl-CoA requirement relieved the auxotrophy.

* Corresponding author. Mailing address: Department of Biology, The University of Michigan, Ann Arbor, MI 48109-1048. Phone: (734) 936-2530. Fax: (734) 647-0884. E-mail: rbender{at}umich.edu.

dagger Present address: Department of Cancer Cell Biology, Harvard School of Public Health, Boston, Mass.

Journal of Bacteriology, April 2001, p. 2709-2714, Vol. 183, No. 8
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.8.2709-2714.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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