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Journal of Bacteriology, May 2001, p. 2746-2754, Vol. 183, No. 9
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.9.2746-2754.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
The ToxR-Mediated Organic Acid Tolerance Response
of Vibrio cholerae Requires OmpU
D. Scott
Merrell,1
Camella
Bailey,2
James B.
Kaper,2 and
Andrew
Camilli1,*
Department of Molecular Biology and
Microbiology, Tufts University School of Medicine, Boston,
Massachusetts 02111,1 and Center for
Vaccine Development, University of Maryland School of Medicine,
Baltimore, Maryland 212012
Received 25 October 2000/Accepted 7 February 2001
It was previously demonstrated that the intestinal pathogen
Vibrio cholerae could undergo an adaptive stress response
known as the acid tolerance response (ATR). The ATR is subdivided into two branches, inorganic ATR and organic ATR. The transcriptional regulator ToxR, while not involved in inorganic ATR, is required for
organic ATR in a ToxT-independent manner. Herein, we investigate the
effect of organic acid stress on global protein synthesis in V. cholerae and show by two-dimensional gel electrophoresis that the
stress response alters the expression of more than 100 polypeptide
species. The expression of more than 20 polypeptide species is altered
in a toxR strain compared to the wild type. Despite this,
ectopic expression of the porin OmpU from an inducible promoter is
shown to be sufficient to bypass the toxR organic ATR
defect. Characterization of the effect of organic acid stress on
ompU and ompT transcription reveals that while
ompU transcription remains virtually unaffected,
ompT transcription is repressed in a ToxR-independent
manner. These transcript levels are similarly reflected in the extent
of accumulation of OmpU and OmpT. Possible roles for OmpU in organic
acid resistance are discussed.
*
Corresponding author. Mailing address: Tufts University
School of Medicine, Department of Molecular Biology and Microbiology, 136 Harrison Ave., Boston, MA 02111. Phone: (617) 636-2144. Fax: (617)
636-0337. E-mail: andrew.camilli{at}tufts.edu.
Journal of Bacteriology, May 2001, p. 2746-2754, Vol. 183, No. 9
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.9.2746-2754.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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