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Journal of Bacteriology, May 2001, p. 2785-2794, Vol. 183, No. 9
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.9.2785-2794.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
The Pyrimidine Operon pyrRPB-carA from
Lactococcus lactis
Jan
Martinussen,*
Jette
Schallert,
Birgit
Andersen, and
Karin
Hammer
Department of Microbiology, The Technical
University of Denmark, DK-2800 Lyngby, Denmark
Received 7 September 2000/Accepted 2 January 2001
The four genes pyrR, pyrP, pyrB, and carA
were found to constitute an operon in Lactococcus lactis
subsp. lactis MG1363. The functions of the different genes
were established by mutational analysis. The first gene in the
operon is the pyrimidine regulatory gene, pyrR, which is
responsible for the regulation of the expression of the pyrimidine
biosynthetic genes leading to UMP formation. The second gene encodes a
membrane-bound high-affinity uracil permease, required for utilization
of exogenous uracil. The last two genes in the operon, pyrB
and carA, encode pyrimidine biosynthetic enzymes; aspartate
transcarbamoylase (pyrB) is the second enzyme in the
pathway, whereas carbamoyl-phosphate synthetase subunit A
(carA) is the small subunit of a heterodimeric enzyme,
catalyzing the formation of carbamoyl phosphate. The carA
gene product is shown to be required for both pyrimidine and arginine
biosynthesis. The expression of the pyrimidine biosynthetic genes
including the pyrRPB-carA operon is subject to control at
the transcriptional level, most probably by an attenuator mechanism in
which PyrR acts as the regulatory protein.
*
Corresponding author. Mailing address: Department of
Microbiology, Building 301, The Technical University of Denmark,
DK-2800 Lyngby, Denmark. Phone: 45 45 25 24 98. Fax: 45 45 88 26 60. E-mail: imjm{at}pop.dtu.dk.
Journal of Bacteriology, May 2001, p. 2785-2794, Vol. 183, No. 9
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.9.2785-2794.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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