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Journal of Bacteriology, May 2001, p. 2803-2807, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2803-2807.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

NreB from Achromobacter xylosoxidans 31A Is a Nickel-Induced Transporter Conferring Nickel Resistance

Gregor Grass,1 Bin Fan,2 Barry P. Rosen,2 Karin Lemke,3 Hans-Günter Schlegel,3 and Christopher Rensing1,*

Department of Soil, Water, and Environmental Science, University of Arizona, Tucson, Arizona 857211; Department of Biochemistry and Molecular Biology, Wayne State University, Detroit, Michigan 482012; and Institut für Mikrobiologie, Georg-August-Universität, 37077 Göttingen, Germany3

Received 13 December 2000/Accepted 14 February 2001

There are two distinct nickel resistance loci on plasmid pTOM9 from Achromobacter xylosoxidans 31A, ncc and nre. Expression of the nreB gene was specifically induced by nickel and conferred nickel resistance on both A. xylosoxidans 31A and Escherichia coli. E. coli cells expressing nreB showed reduced accumulation of Ni2+, suggesting that NreB mediated nickel efflux. The histidine-rich C-terminal region of NreB was not essential but contributed to maximal Ni2+ resistance.

* Corresponding author. Mailing address: Department of Soil, Water, and Environmental Science, University of Arizona, Shantz Bldg. #38, Rm. 429, Tucson, AZ 85721. Phone: (520) 626-8482. Fax: (520) 621-1647. E-mail: rensingc{at}ag.arizona.edu.

Journal of Bacteriology, May 2001, p. 2803-2807, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2803-2807.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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