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Journal of Bacteriology, May 2001, p. 2937-2942, Vol. 183, No. 9
School of Biosciences, University of
Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom
Received 1 November 2000/Accepted 31 January 2001
Three potential regulators of flagellar expression present in the
genome sequence of Campylobacter jejuni NCTC 11168, the genes rpoN, flgR, and
fliA, which encode the alternative sigma factor
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.9.2937-2942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Roles of rpoN, fliA,
and flgR in Expression of Flagella in
Campylobacter jejuni
54, the
54-associated transcriptional
activator FlgR, and the flagellar sigma factor
28,
respectively, were investigated for their role in global regulation of
flagellar expression. The three genes were insertionally inactivated in
C. jejuni strains NCTC 11168 and NCTC 11828. Electron
microscopic studies of the wild-type and mutant strains showed that the
rpoN and flgR mutants were nonflagellate
and that the fliA mutant had truncated flagella.
Immunoblotting experiments with the three mutants confirmed the roles
of rpoN, flgR, and fliA in
the expression of flagellin.
*
Corresponding author. Mailing address: School of
Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT,
United Kingdom. Phone: 44(0) 121-414-6562. Fax: 44(0) 121-414-5925. E-mail: c.w.penn{at}bham.ac.uk.
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