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Journal of Bacteriology, May 2001, p. 2937-2942, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2937-2942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Roles of rpoN, fliA, and flgR in Expression of Flagella in Campylobacter jejuni

Aparna Jagannathan, Chrystala Constantinidou, and Charles W. Penn*

School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom

Received 1 November 2000/Accepted 31 January 2001

Three potential regulators of flagellar expression present in the genome sequence of Campylobacter jejuni NCTC 11168, the genes rpoN, flgR, and fliA, which encode the alternative sigma factor sigma 54, the sigma 54-associated transcriptional activator FlgR, and the flagellar sigma factor sigma 28, respectively, were investigated for their role in global regulation of flagellar expression. The three genes were insertionally inactivated in C. jejuni strains NCTC 11168 and NCTC 11828. Electron microscopic studies of the wild-type and mutant strains showed that the rpoN and flgR mutants were nonflagellate and that the fliA mutant had truncated flagella. Immunoblotting experiments with the three mutants confirmed the roles of rpoN, flgR, and fliA in the expression of flagellin.

* Corresponding author. Mailing address: School of Biosciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom. Phone: 44(0) 121-414-6562. Fax: 44(0) 121-414-5925. E-mail: c.w.penn{at}bham.ac.uk.

Journal of Bacteriology, May 2001, p. 2937-2942, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2937-2942.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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