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Journal of Bacteriology, May 2001, p. 2952-2956, Vol. 183, No. 9
National Institute of Genetics, Department of
Molecular Genetics, Mishima, Shizuoka 411-8540, Japan
Received 21 December 2000/Accepted 20 February 2001
Rsd (regulator of sigma D) is an anti-sigma factor for the
Escherichia coli RNA polymerase
0021-9193/01/$04.00+0 DOI: 10.1128/JB.183.9.2952-2956.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Mapping of the Rsd Contact Site on the Sigma 70 Subunit of Escherichia coli RNA Polymerase

and
70 subunit.
The contact site of Rsd on
70 was analyzed after mapping
of the contact-dependent cleavage sites by Rsd-tethered
iron-p-bromoacetamidobenzyl EDTA and by analysis of the
complex formation between Ala-substituted
70 and Rsd.
Results indicate that the Rsd contact site is located downstream of the
promoter
35 recognition helix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core
enzyme and
70 contact transcription factors.
*
Corresponding author. Mailing address: National
Institute of Genetics, Department of Molecular Genetics, Mishima,
Shizuoka 411-8540, Japan. Phone: (81) 559-81-6741. Fax: (81)
559-81-6746. E-mail: aishiham{at}lab.nig.ac.jp.
Present address: Cancer Institute, Toshima-ku, Tokyo 170-8455, Japan.
On leave of absence from Biophysics Division, Saha Institute of
Nuclear Physics, Calcutta-700 037, India.
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