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Journal of Bacteriology, May 2001, p. 2952-2956, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2952-2956.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mapping of the Rsd Contact Site on the Sigma 70 Subunit of Escherichia coli RNA Polymerase

Miki Jishage,dagger Dipak Dasgupta,Dagger and Akira Ishihama*

National Institute of Genetics, Department of Molecular Genetics, Mishima, Shizuoka 411-8540, Japan

Received 21 December 2000/Accepted 20 February 2001

Rsd (regulator of sigma D) is an anti-sigma factor for the Escherichia coli RNA polymerase sigma 70 subunit. The contact site of Rsd on sigma 70 was analyzed after mapping of the contact-dependent cleavage sites by Rsd-tethered iron-p-bromoacetamidobenzyl EDTA and by analysis of the complex formation between Ala-substituted sigma 70 and Rsd. Results indicate that the Rsd contact site is located downstream of the promoter -35 recognition helix-turn-helix motif within region 4, overlapping with the regions involved in interaction with both core enzyme and sigma 70 contact transcription factors.

* Corresponding author. Mailing address: National Institute of Genetics, Department of Molecular Genetics, Mishima, Shizuoka 411-8540, Japan. Phone: (81) 559-81-6741. Fax: (81) 559-81-6746. E-mail: aishiham{at}lab.nig.ac.jp.

dagger Present address: Cancer Institute, Toshima-ku, Tokyo 170-8455, Japan.

Dagger On leave of absence from Biophysics Division, Saha Institute of Nuclear Physics, Calcutta-700 037, India.

Journal of Bacteriology, May 2001, p. 2952-2956, Vol. 183, No. 9
0021-9193/01/$04.00+0   DOI: 10.1128/JB.183.9.2952-2956.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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