This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Martínez-Argudo, I.
Right arrow Articles by Contreras, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Martínez-Argudo, I.
Right arrow Articles by Contreras, A.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2002, p. 200-206, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.200-206.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Domain Interactions on the ntr Signal Transduction Pathway: Two-Hybrid Analysis of Mutant and Truncated Derivatives of Histidine Kinase NtrB

Isabel Martínez-Argudo, Paloma Salinas, Rafael Maldonado, and Asunción Contreras*

División de Genética, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain

Received 27 July 2001/ Accepted 9 October 2001

We have used the yeast two-hybrid system to analyze protein-protein interactions mediated by domains of regulatory proteins of the ntr signal transduction system, including interactions among NtrB derivatives and their interactions with NtrC and PII from Klebsiella pneumoniae. Interactions took place only between proteins or protein domains belonging to the ntr signal transduction system and not between proteins or domains from noncognate regulators. NtrB and its transmitter domain, but not NtrC, CheA, or the cytoplasmic C terminus of EnvZ, interacted with PII. In addition, interaction of NtrB with NtrC, but not with PII, depended on the histidine phosphotransfer domain. Point mutation A129T, diminishing the NtrC phosphatase activity of NtrB, affected the strength of the signals between NtrC and the transmitter module of NtrB but had no impact on PII signals, suggesting that A129T prevents the conformational change needed by NtrB to function as a phosphatase for NtrC, rather than disturbing binding to PII.

* Corresponding author. Mailing address: División de Genética, Facultad de Ciencias, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain. Phone: 34 96 590 3957. Fax: 34 96 590 9569. Email: contrera{at}ua.es.

Journal of Bacteriology, January 2002, p. 200-206, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.200-206.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ruiz, D., Salinas, P., Lopez-Redondo, M. L., Cayuela, M. L., Marina, A., Contreras, A. (2008). Phosphorylation-independent activation of the atypical response regulator NblR. Microbiology 154: 3002-3015 [Abstract] [Full Text]  
  • Xie, Z., Dou, Y., Ping, S., Chen, M., Wang, G., Elmerich, C., Lin, M. (2006). Interaction between NifL and NifA in the nitrogen-fixing Pseudomonas stutzeri A1501. Microbiology 152: 3535-3542 [Abstract] [Full Text]  
  • Seok, J.-S., Kaplan, S., Oh, J.-I. (2006). Interacting specificity of a histidine kinase and its cognate response regulator: the PrrBA system of Rhodobacter sphaeroides.. Microbiology 152: 2479-2490 [Abstract] [Full Text]  
  • Zhu, Y., Conrad, M. C., Zhang, Y., Roberts, G. P. (2006). Identification of Rhodospirillum rubrum GlnB Variants That Are Altered in Their Ability To Interact with Different Targets in Response to Nitrogen Status Signals.. J. Bacteriol. 188: 1866-1874 [Abstract] [Full Text]  
  • Ramos, J. L., Martinez-Bueno, M., Molina-Henares, A. J., Teran, W., Watanabe, K., Zhang, X., Gallegos, M. T., Brennan, R., Tobes, R. (2005). The TetR Family of Transcriptional Repressors. Microbiol. Mol. Biol. Rev. 69: 326-356 [Abstract] [Full Text]  
  • Wise, A. A., Voinov, L., Binns, A. N. (2005). Intersubunit Complementation of Sugar Signal Transduction in VirA Heterodimers and Posttranslational Regulation of VirA Activity in Agrobacterium tumefaciens. J. Bacteriol. 187: 213-223 [Abstract] [Full Text]  
  • Burillo, S., Luque, I., Fuentes, I., Contreras, A. (2004). Interactions between the Nitrogen Signal Transduction Protein PII and N-Acetyl Glutamate Kinase in Organisms That Perform Oxygenic Photosynthesis. J. Bacteriol. 186: 3346-3354 [Abstract] [Full Text]  
  • Elsen, S., Duche, O., Colbeau, A. (2003). Interaction between the H2 Sensor HupUV and the Histidine Kinase HupT Controls HupSL Hydrogenase Synthesis in Rhodobacter capsulatus. J. Bacteriol. 185: 7111-7119 [Abstract] [Full Text]  
  • Pawlowski, A., Riedel, K.-U., Klipp, W., Dreiskemper, P., Gross, S., Bierhoff, H., Drepper, T., Masepohl, B. (2003). Yeast Two-Hybrid Studies on Interaction of Proteins Involved in Regulation of Nitrogen Fixation in the Phototrophic Bacterium Rhodobacter capsulatus. J. Bacteriol. 185: 5240-5247 [Abstract] [Full Text]  
  • Pioszak, A. A., Ninfa, A. J. (2003). Genetic and Biochemical Analysis of Phosphatase Activity of Escherichia coli NRII (NtrB) and Its Regulation by the PII Signal Transduction Protein. J. Bacteriol. 185: 1299-1315 [Abstract] [Full Text]  
  • Porter, S. W., Xu, Q., West, A. H. (2003). Ssk1p Response Regulator Binding Surface on Histidine- Containing Phosphotransfer Protein Ypd1p. Eukaryot Cell 2: 27-33 [Abstract] [Full Text]  
  • Martinez-Argudo, I., Contreras, A. (2002). PII T-Loop Mutations Affecting Signal Transduction to NtrB Also Abolish Yeast Two-Hybrid Interactions. J. Bacteriol. 184: 3746-3748 [Abstract] [Full Text]  
  • Little, R., Colombo, V., Leech, A., Dixon, R. (2002). Direct Interaction of the NifL Regulatory Protein with the GlnK Signal Transducer Enables the Azotobacter vinelandii NifL-NifA Regulatory System to Respond to Conditions Replete for Nitrogen. J. Biol. Chem. 277: 15472-15481 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»