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Journal of Bacteriology, January 2002, p. 216-223, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.216-223.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Degradation of Aromatics and Chloroaromatics by Pseudomonas sp. Strain B13: Cloning, Characterization, and Analysis of Sequences Encoding 3-Oxoadipate:Succinyl-Coenzyme A (CoA) Transferase and 3-Oxoadipyl-CoA Thiolase

Markus Göbel, Kerstin Kassel-Cati,,{dagger} Eberhard Schmidt, and Walter Reineke*

Chemische Mikrobiologie, Bergische Universität-Gesamthochschule Wuppertal, Wuppertal, Germany

Received 16 July 2001/ Accepted 2 October 2001

3-Oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase carry out the ultimate steps in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates in bacteria utilizing the 3-oxoadipate pathway. This report describes the characterization of DNA fragments with the overall length of 5.9 kb from Pseudomonas sp. strain B13 that encode these enzymes. DNA sequence analysis revealed five open reading frames (ORFs) plus an incomplete one. ORF1, of unknown function, has a length of 414 bp. ORF2 (catI) encodes a polypeptide of 282 amino acids and starts at nucleotide 813. ORF3 (catJ) encodes a polypeptide of 260 amino acids and begins at nucleotide 1661. CatI and CatJ are the subunits of the 3-oxoadipate:succinyl-CoA transferase, whose activity was demonstrated when both genes were ligated into expression vector pET11a. ORF4, termed catF, codes for a protein of 401 amino acid residues with a predicted mass of 41,678 Da with 3-oxoadipyl-CoA thiolase activity. The last three ORFs seem to form an operon since they are oriented in the same direction and showed an overlapping of 1 bp between catI and catJ and of 4 bp between catJ and catF. Conserved functional groups important for the catalytic activity of CoA transferases and thiolases were identified in CatI, CatJ, and CatF. ORF5 (catD) encodes the 3-oxoadipate enol-lactone hydrolase. An incomplete ORF6 of 1,183 bp downstream of ORF5 and oriented in the opposite direction was found. The protein sequence deduced from ORF6 showed a putative AMP-binding domain signature.

* Corresponding author. Mailing address: Bergische Universität-Gesamthochschule Wuppertal, Chemische Mikrobiologie, Fachbereich 9, Gaußstraße 20, D-42097 Wuppertal, Germany. Phone: 49-202-4392456. Fax: 49-202-4392698. E-mail: reineke{at}uni-wuppertal.de.

{dagger} Present address: pc-plus Computing GmbH, München, Germany.

Journal of Bacteriology, January 2002, p. 216-223, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.216-223.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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