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Journal of Bacteriology, January 2002, p. 323-326, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.323-326.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

WbjA Adds Glucose To Complete the O-Antigen Trisaccharide Repeating Unit of the Lipopolysaccharide of Pseudomonas aeruginosa Serogroup O11

Charles R. Dean,1,{dagger} Anup Datta,2 Russell W. Carlson,2 and Joanna B. Goldberg1*

Department of Microbiology, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908,1 Complex Carbohydrate Research Center, University of Georgia, Athens, Georgia 306022

Received 20 August 2001/ Accepted 20 September 2001

Lipopolysaccharide from a wbjA mutant, deficient in a putative glycosyltransferase from Pseudomonas aeruginosa serogroup O11, was compared to that from an O-antigen polymerase mutant. Results suggest that WbjA adds the terminal glucose to complete the serogroup O11 O-antigen unit and identifies the biological repeating unit as [-2)-ß-D-glucose-(1-3)-{alpha}-L-N-acetylfucosamine-(1-3)-ß-D-N-acetylfucosamine-(1].


* Corresponding author. Mailing address: Department of Microbiology, University of Virginia Health Sciences Center, Box 800734, Charlottesville, VA 22908. Phone: (434) 243-2774. Fax: (434) 982-1071. E-mail: jbg2b{at}virginia.edu.

{dagger} Present address: Wyeth-Ayerst Research, Pearl River, NY 10965.


Journal of Bacteriology, January 2002, p. 323-326, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.323-326.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




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