This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Paterson, E. S.
Right arrow Articles by Lambert, I. B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Paterson, E. S.
Right arrow Articles by Lambert, I. B.

 Previous Article  |  Next Article 

Journal of Bacteriology, January 2002, p. 51-58, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.51-58.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Regulation of the nfsA Gene in Escherichia coli by SoxS

E. Suzanne Paterson, Sherri E. Boucher,,{dagger} and I. B. Lambert*

Department of Biology, Carleton University,Ottawa, Ontario K1S 5B6, Canada

Received 21 June 2001/ Accepted 3 October 2001

In Escherichia coli, the response to oxidative stress due to elevated levels of superoxide is mediated, in part, by the soxRS regulon. One member of the soxRS regulon, nfsA, encodes the major oxygen-insensitive nitroreductase in Escherichia coli which catalyzes the reduction of nitroaromatic and nitroheterocyclic compounds by NADPH. In this study we investigate the regulation of nfsA in response to the superoxide generating compound paraquat. The transcription start site (TSS) of nfsA was located upstream of the ybjC gene, a small open reading frame of unknown function located directly upstream of nfsA, suggesting that these two genes form an operon. The activity of the promoter associated with this TSS was confirmed with lacZ fusions and was shown to be inducible by paraquat. Footprinting and band shift analysis showed that purified His-tagged SoxS protein binds to a 20-base sequence 10 bases upstream of the -35 promoter sequence in the forward orientation, suggesting that the ybjC-nfsA promoter is a class I SoxS-dependent promoter.

* Corresponding author. Mailing address: Department of Biology, Carleton University, 1125 Colonel By Drive, Ottawa, Ontario K1S 5B6, Canada. Phone: (613) 520-2600, x3893. Fax: (613) 520-2569. E-mail: ilambert{at}ccs.carleton.ca.

{dagger} Present address: Department of Biochemistry, Microbiology, and Immunology, University of Ottawa, Ottawa, Ontario K1H 8M5, Canada.

Journal of Bacteriology, January 2002, p. 51-58, Vol. 184, No. 1
0021-9193/01/$04.00+0     DOI: 10.1128/JB.184.1.51-58.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Keeney, D., Ruzin, A., McAleese, F., Murphy, E., Bradford, P. A. (2008). MarA-mediated overexpression of the AcrAB efflux pump results in decreased susceptibility to tigecycline in Escherichia coli. J Antimicrob Chemother 61: 46-53 [Abstract] [Full Text]  
  • Bore, E., Hebraud, M., Chafsey, I., Chambon, C., Skjaeret, C., Moen, B., Moretro, T., Langsrud, O., Rudi, K., Langsrud, S. (2007). Adapted tolerance to benzalkonium chloride in Escherichia coli K-12 studied by transcriptome and proteome analyses. Microbiology 153: 935-946 [Abstract] [Full Text]  
  • Salamanca-Pinzon, S.G., Camacho-Carranza, R., Hernandez-Ojeda, S.L., Espinosa-Aguirre, J.J. (2006). Nitrocompound activation by cell-free extracts of nitroreductase-proficient Salmonella typhimurium strains. Mutagenesis 21: 369-374 [Abstract] [Full Text]  
  • Perez-Reinado, E., Blasco, R., Castillo, F., Moreno-Vivian, C., Roldan, M. D. (2005). Regulation and Characterization of Two Nitroreductase Genes, nprA and nprB, of Rhodobacter capsulatus. Appl. Environ. Microbiol. 71: 7643-7649 [Abstract] [Full Text]  
  • Streker, K., Freiberg, C., Labischinski, H., Hacker, J., Ohlsen, K. (2005). Staphylococcus aureus NfrA (SA0367) Is a Flavin Mononucleotide-Dependent NADPH Oxidase Involved in Oxidative Stress Response. J. Bacteriol. 187: 2249-2256 [Abstract] [Full Text]  
  • van Dijk, K., Nelson, E. B. (2000). Fatty Acid Competition as a Mechanism by Which Enterobacter cloacae Suppresses Pythium ultimum Sporangium Germination and Damping-Off. Appl. Environ. Microbiol. 66: 5340-5347 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»