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Journal of Bacteriology, May 2002, p. 2767-2779, Vol. 184, No. 10
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.10.2767-2779.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

TraG-Like Proteins of DNA Transfer Systems and of the Helicobacter pylori Type IV Secretion System: Inner Membrane Gate for Exported Substrates?

Gunnar Schröder,1 Sabine Krause,1 Ellen L. Zechner,2 Beth Traxler,3 Hye-Jeong Yeo,4 Rudi Lurz,1 Gabriel Waksman,4 and Erich Lanka1*

Max-Planck-Institut für Molekulare Genetik, Dahlem, D-14195 Berlin, Germany,1 Institut für Molekularbiologie, Biochemie und Mikrobiologie, Karl-Franzens-Universität Graz, A-8010 Graz, Austria,2 Department of Genetics, University of Washington, Seattle, Washington 98195,3 Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, Missouri 631104

Received 13 December 2001/ Accepted 16 February 2002

TraG-like proteins are potential NTP hydrolases (NTPases) that are essential for DNA transfer in bacterial conjugation. They are thought to mediate interactions between the DNA-processing (Dtr) and the mating pair formation (Mpf) systems. TraG-like proteins also function as essential components of type IV secretion systems of several bacterial pathogens such as Helicobacter pylori. Here we present the biochemical characterization of three members of the family of TraG-like proteins, TraG (RP4), TraD (F), and HP0524 (H. pylori). These proteins were found to have a pronounced tendency to form oligomers and were shown to bind DNA without sequence specificity. Standard NTPase assays indicated that these TraG-like proteins do not possess postulated NTP-hydrolyzing activity. Surface plasmon resonance was used to demonstrate an interaction between TraG and relaxase TraI of RP4. Topology analysis of TraG revealed that TraG is a transmembrane protein with cytosolic N and C termini and a short periplasmic domain close to the N terminus. We predict that multimeric inner membrane protein TraG forms a pore. A model suggesting that the relaxosome binds to the TraG pore via TraG-DNA and TraG-TraI interactions is presented.

* Corresponding author. Mailing address: Max-Planck-Institut für Molekulare Genetik, Abteilung Lehrach, Ihnestraße 73, Dahlem, D-14195 Berlin, Germany. Phone: 49 30 8413 1696. Fax: 49 30 8413 1130. E-mail: lanka{at}molgen.mpg.de.

Journal of Bacteriology, May 2002, p. 2767-2779, Vol. 184, No. 10
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.10.2767-2779.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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