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Journal of Bacteriology, May 2002, p. 2841-2844, Vol. 184, No. 10
0021-9193/02/$04.00+0 DOI: 10.1128/JB.184.10.2841-2844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado 80523-1177
Received 1 November 2001/ Accepted 20 February 2002
Contour-clamped homogeneous electric field gel analysis of genomic DNA of the plant pathogen Clavibacter michiganensis subsp. sepedonicus revealed the presence of a previously unreported extrachromosomal element. This new element was demonstrated to be a linear plasmid. Of 11 strains evaluated, all contained either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.
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