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Journal of Bacteriology, May 2002, p. 2841-2844, Vol. 184, No. 10
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.10.2841-2844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Linear Plasmid in the Genome of Clavibacter michiganensis subsp. sepedonicus

Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado 80523-1177

Received 1 November 2001/ Accepted 20 February 2002

Contour-clamped homogeneous electric field gel analysis of genomic DNA of the plant pathogen Clavibacter michiganensis subsp. sepedonicus revealed the presence of a previously unreported extrachromosomal element. This new element was demonstrated to be a linear plasmid. Of 11 strains evaluated, all contained either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.

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