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Journal of Bacteriology, May 2002, p. 2841-2844, Vol. 184, No. 10
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.10.2841-2844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Linear Plasmid in the Genome of Clavibacter michiganensis subsp. sepedonicus

Susan E. Brown, Dennis L. Knudson, and Carol A. Ishimaru*

Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, Colorado 80523-1177

Received 1 November 2001/ Accepted 20 February 2002

Contour-clamped homogeneous electric field gel analysis of genomic DNA of the plant pathogen Clavibacter michiganensis subsp. sepedonicus revealed the presence of a previously unreported extrachromosomal element. This new element was demonstrated to be a linear plasmid. Of 11 strains evaluated, all contained either a 90-kb (pCSL1) or a 140-kb (pCSL2) linear plasmid.

* Corresponding author. Mailing address: Department of Bioagricultural Sciences and Pest Management, Colorado State University, Fort Collins, CO 80523-1177.

Journal of Bacteriology, May 2002, p. 2841-2844, Vol. 184, No. 10
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.10.2841-2844.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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