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Journal of Bacteriology, May 2002, p. 2845-2849, Vol. 184, No. 10
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.10.2845-2849.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Ribonuclease M5 Has Few, If Any, mRNA Substrates in Bacillus subtilis

Ciarán Condon,* Jordi Rourera, Dominique Brechemier-Baey, and Harald Putzer

UPR 9073, Institut de Biologie Physico-Chimique, 75005 Paris, France

Received 10 December 2001/ Accepted 21 February 2002

In Bacillus subtilis, maturation of 5S rRNA is catalyzed by an enzyme called RNase M5. We searched for potential mRNA substrates for RNase M5 by gene array technology, based on the premise that most endonucleolytic cleavages have an effect on the stability of RNA and hence on steady-state levels of expression. Only a handful of genes had significantly altered expression in rnmV mutants compared to wild-type strains that could subsequently be confirmed by Northern blotting. The effect of RNase M5 on the expression of the best candidates, the odhAB and sucCD operons, is indirect, by a mechanism we do not yet understand. We show that an effect of RNase M5 on the expression of the remaining candidate, ctsR, is due to the failure to process the 5S rRNA contained in the rrnW lying directly upstream. We thus conclude that RNase M5 has very few or possibly no mRNA substrates in B. subtilis.

* Corresponding author. Mailing address: UPR 9073, Institut de Biologie Physico-Chimique, 13 rue Pierre et Marie Curie, 75005 Paris, France. Phone: (33)-1-58 41 51 23. Fax: (33)-1-58 41 50 20. E-mail: condon{at}ibpc.fr.

Journal of Bacteriology, May 2002, p. 2845-2849, Vol. 184, No. 10
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.10.2845-2849.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Allemand, F., Mathy, N., Brechemier-Baey, D., Condon, C. (2005). The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member. Nucleic Acids Res 33: 4368-4376 [Abstract] [Full Text]  
  • Ohki, R., Tateno, K. (2004). Increased Stability of bmr3 mRNA Results in a Multidrug-Resistant Phenotype in Bacillus subtilis. J. Bacteriol. 186: 7450-7455 [Abstract] [Full Text]  
  • Condon, C. (2003). RNA Processing and Degradation in Bacillus subtilis. Microbiol. Mol. Biol. Rev. 67: 157-174 [Abstract] [Full Text]  
  • Meinken, C., Blencke, H.-M., Ludwig, H., Stulke, J. (2003). Expression of the glycolytic gapA operon in Bacillus subtilis: differential syntheses of proteins encoded by the operon. Microbiology 149: 751-761 [Abstract] [Full Text]  


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