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Journal of Bacteriology, June 2002, p. 3122-3125, Vol. 184, No. 11
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.11.3122-3125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding

Sukanya Narasimhan,1 Felix Santiago,1 Raymond A. Koski,2 Brandon Brei,3 John F. Anderson,4 Durland Fish,3 and Erol Fikrig1*

Section of Rheumatology, Department of Internal Medicine,1 Department of Epidemiology and Public Health, Yale University School of Medicine,3 L Diagnostics, New Haven, Connecticut 06520,2 Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, Connecticut 065044

Received 2 November 2001/ Accepted 14 February 2002

Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasmic proteins were preferentially expressed in engorging ticks. These data demonstrate a new approach to the global analysis of B. burgdorferi genes that are preferentially expressed within the vector during feeding.

* Corresponding author. Mailing address: 608 Laboratory of Clinical Investigation, Section of Rheumatology, Department of Internal Medicine, Yale University School of Medicine, 333 Cedar St., New Haven, CT 06520-8031. Phone: (203) 785-2453. Fax: (203) 785-7053. E-mail: erol.fikrig{at}yale.edu.

Journal of Bacteriology, June 2002, p. 3122-3125, Vol. 184, No. 11
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.11.3122-3125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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