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Journal of Bacteriology, June 2002, p. 3122-3125, Vol. 184, No. 11
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.11.3122-3125.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Examination of the Borrelia burgdorferi Transcriptome in Ixodes scapularis during Feeding

Section of Rheumatology, Department of Internal Medicine,¹ Department of Epidemiology and Public Health, Yale University School of Medicine,³ L Diagnostics, New Haven, Connecticut 06520,² Department of Entomology, Connecticut Agricultural Experiment Station, New Haven, Connecticut 06504⁴

Received 2 November 2001/ Accepted 14 February 2002

Borrelia burgdorferi gene expression within the guts of engorging Ixodes scapularis ticks was examined by use of differential immunoscreening and differential expression with a customized amplified library. Fourteen chromosomal genes involved in energy metabolism, substrate transport, and signal transduction and 10 (4 chromosomal and 6 plasmid) genes encoding putative lipoproteins and periplasmic proteins were preferentially expressed in engorging ticks. These data demonstrate a new approach to the global analysis of B. burgdorferi genes that are preferentially expressed within the vector during feeding.

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