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Journal of Bacteriology, June 2002, p. 3142-3145, Vol. 184, No. 11
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.11.3142-3145.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Behavior of Sister Copies of Mini-F Plasmid after Synchronized Plasmid Replication in Escherichia coli Cells

Toshinari Onogi,1 Takeyoshi Miki,2 and Sota Hiraga1*

Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kuhonji 4-24-1, Kumamoto 862-0976,1 Department of Molecular Microbiology, Graduate School of Pharmaceutical Sciences, Kyuushu University, 3-1 Maidashi, Higashi-ku, Fukuoka 812-8582, Japan2

Received 21 November 2001/ Accepted 27 February 2002

To clarify whether sister copies of mini-F plasmid are immediately separated from each other after replication, we analyzed the behavior of sister mini-F copies after synchronized replication of mini-F. Sister copies of mini-F were separated immediately or shortly after replication, in contrast to sister oriC copies of the Escherichia coli chromosome.

* Corresponding author. Mailing address: Department of Molecular Cell Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kuhonji 4-24-1, Kumamoto 862-0976, Japan. Phone: 81-96-373-6578. Fax: 81-96-373-6582. E-mail: hiraga{at}gpo.kumamoto-u.ac.jp.

Journal of Bacteriology, June 2002, p. 3142-3145, Vol. 184, No. 11
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.11.3142-3145.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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