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Journal of Bacteriology, June 2002, p. 3214-3223, Vol. 184, No. 12
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.12.3214-3223.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Up-Regulation of the Yersinia enterocolitica yop Regulon by Deletion of the Flagellum Master Operon flhDC

Sophie Bleves,1,2,{dagger} Marie-Noëlle Marenne,1,2 Gautier Detry,1,2 and Guy R. Cornelis1,2,3*

Christian de Duve Institute of Cellular Pathology,,1 Faculté de Médecine, Université de Louvain, B-1200 Brussels, Belgium,2 Molekulare Mikrobiologie, Biozentrum der Universität Basel, CH-4056 Basel, Switzerland3

Received 26 March 2001/ Accepted 13 March 2002

The Yop virulon enables extracellularly located Yersinia, in close contact with a eukaryotic target cell, to inject bacterial toxic proteins directly into the cytosol of this cell. Several Ysc proteins, forming the Yop secretion apparatus, display homology with proteins of the flagellar basal body. To determine whether this relationship could extend to the regulatory pathways, we analyzed the influence of flhDC, the master regulatory operon of the flagellum, on the yop regulon. In an flhDC mutant, the yop regulon was up-regulated. The transcription of virF and the steady-state level of the transcriptional activator VirF were enhanced. yop transcription was increased at 37°C and could also be detected at a low temperature. Yop secretion was increased at 37°C and occurred even at a low temperature. The Ysc secretion machinery was thus functional at room temperature in the absence of flagella, implying that in wild-type bacteria, FlhD and/or FlhC, or the product of a gene downstream of flhDC, represses the yop regulon. In agreement with this notion, increased expression of flhDC in wild-type bacteria resulted in the oversecretion of flagellins at room temperature and in decreased Yop secretion at 37°C.

* Corresponding author. Mailing address: Molekulare Mikrobiologie, Biozentrum der Universität Basel, Klingelbergstr. 50-70, CH-4056 Basel, Switzerland. Phone: 41 61 267 31 11. Fax: 41 61 267 21 18. E-mail: guy.cornelis{at}unibas.ch.

{dagger} Present address: Laboratoire d'Ingéniérie des Systèmes Macromoléculaires, UPR9027, IBSM/CNRS, 13402 Marseille Cedex 20, France.

Journal of Bacteriology, June 2002, p. 3214-3223, Vol. 184, No. 12
0021-9193/02/$04.00+0     DOI: 10.1128/JB.184.12.3214-3223.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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