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Journal of Bacteriology, June 2002, p. 3416-3418, Vol. 184, No. 12
0021-9193/02/$04.00+0 DOI: 10.1128/JB.184.12.3416-3418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
Requirement for NusG for Transcription Antitermination In Vivo by the
N Protein
Ying Zhou,1 Joshua J. Filter,1 Donald L. Court,2 Max E. Gottesman,3 and David I. Friedman1*
Department of Microbiology and Immunology, The University of Michigan, Medical School, Ann Arbor, Michigan 48109-0620,1
Gene Regulation and Chromosome Biology Laboratory, Division of Basic Sciences, National Cancer Institute at Frederick, Frederick, Maryland 21702,2
Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, New York 100323
Received 25 January 2002/
Accepted 24 March 2002
Transcription antitermination by the bacteriophage
N protein is stimulated in vitro by the Escherichia coli NusG protein. Earlier work suggested that NusG was not required for N activity in vivo. Here we present evidence that NusG also stimulates N-mediated transcription antitermination in intact cells.
* Corresponding author. Mailing address: Department of Microbiology and Immunology, University of Michigan, 5641 Medical Science Bldg. II, Ann Arbor, MI 48109-0620. Phone: (734) 769-0181. Fax: (734) 764-3562. E-mail: davidfri{at}umich.edu.
Journal of Bacteriology, June 2002, p. 3416-3418, Vol. 184, No. 12
0021-9193/02/$04.00+0 DOI: 10.1128/JB.184.12.3416-3418.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.
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Copyright © 2002 by the American Society for Microbiology. All rights reserved.