This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Suyama, A. Articles by Furukawa, K. Search for Related Content PubMed PubMed Citation Articles by Suyama, A. Articles by Furukawa, K.

Molecular Characterization of the PceA Reductive Dehalogenase of Desulfitobacterium sp. Strain Y51

Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581,¹ Towakagaku Co., Ltd., Hiroshima 730-0841, Japan²

Received 22 January 2002/ Accepted 4 April 2002

The tetrachloroethene (PCE) reductive dehalogenase (encoded by the pceA gene and designated PceA dehalogenase) of Desulfitobacterium sp. strain Y51 was purified and characterized. The expression of the enzyme was highly induced in the presence of PCE and trichloroethene (TCE). The purified enzyme catalyzed the reductive dehalogenation of PCE via TCE to cis-1,2-dichloroethene at a specific activity of 113.6 nmol · min^-1 · mg of protein^-1. The apparent K_m values for PCE and TCE were 105.7 and 535.3 µM, respectively. Chlorinated ethenes other than PCE and TCE were not dehalogenated. However, the enzyme exhibited dehalogenation activity for various chlorinated ethanes such as hexachloroethane, pentachloroethane, 1,1,1,2-tetrachloroethane, and 1,1,2,2-tetrachloroethane. The pceA gene of Desulfitobacterium sp. strain Y51 was identified in a 2.8-kb DNA fragment and used to express the protein in Escherichia coli for the preparation of antibodies. Immunoblot analyses located PceA in the periplasm of the cell.

This article has been cited by other articles:

• Marzorati, M., de Ferra, F., Van Raemdonck, H., Borin, S., Allifranchini, E., Carpani, G., Serbolisca, L., Verstraete, W., Boon, N., Daffonchio, D. (2007). A Novel Reductive Dehalogenase, Identified in a Contaminated Groundwater Enrichment Culture and in Desulfitobacterium dichloroeliminans Strain DCA1, Is Linked to Dehalogenation of 1,2-Dichloroethane. Appl. Environ. Microbiol. 73: 2990-2999 [Abstract] [Full Text]  
• Futagami, T., Yamaguchi, T., Nakayama, S.-i., Goto, M., Furukawa, K. (2006). Effects of Chloromethanes on Growth of and Deletion of the pce Gene Cluster in Dehalorespiring Desulfitobacterium hafniense Strain Y51. Appl. Environ. Microbiol. 72: 5998-6003 [Abstract] [Full Text]  
• Nonaka, H., Keresztes, G., Shinoda, Y., Ikenaga, Y., Abe, M., Naito, K., Inatomi, K., Furukawa, K., Inui, M., Yukawa, H. (2006). Complete Genome Sequence of the Dehalorespiring Bacterium Desulfitobacterium hafniense Y51 and Comparison with Dehalococcoides ethenogenes 195. J. Bacteriol. 188: 2262-2274 [Abstract] [Full Text]  
• Waller, A. S., Krajmalnik-Brown, R., Loffler, F. E., Edwards, E. A. (2005). Multiple Reductive-Dehalogenase-Homologous Genes Are Simultaneously Transcribed during Dechlorination by Dehalococcoides-Containing Cultures. Appl. Environ. Microbiol. 71: 8257-8264 [Abstract] [Full Text]  
• Johnson, D. R., Lee, P. K. H., Holmes, V. F., Fortin, A. C., Alvarez-Cohen, L. (2005). Transcriptional Expression of the tceA Gene in a Dehalococcoides-Containing Microbial Enrichment. Appl. Environ. Microbiol. 71: 7145-7151 [Abstract] [Full Text]  
• Krajmalnik-Brown, R., Holscher, T., Thomson, I. N., Saunders, F. M., Ritalahti, K. M., Loffler, F. E. (2004). Genetic Identification of a Putative Vinyl Chloride Reductase in Dehalococcoides sp. Strain BAV1. Appl. Environ. Microbiol. 70: 6347-6351 [Abstract] [Full Text]  
• Holscher, T., Krajmalnik-Brown, R., Ritalahti, K. M., von Wintzingerode, F., Gorisch, H., Loffler, F. E., Adrian, L. (2004). Multiple Nonidentical Reductive-Dehalogenase-Homologous Genes Are Common in Dehalococcoides. Appl. Environ. Microbiol. 70: 5290-5297 [Abstract] [Full Text]  
• Thibodeau, J., Gauthier, A., Duguay, M., Villemur, R., Lepine, F., Juteau, P., Beaudet, R. (2004). Purification, Cloning, and Sequencing of a 3,5-Dichlorophenol Reductive Dehalogenase from Desulfitobacterium frappieri PCP-1. Appl. Environ. Microbiol. 70: 4532-4537 [Abstract] [Full Text]  
• Muller, J. A., Rosner, B. M., von Abendroth, G., Meshulam-Simon, G., McCarty, P. L., Spormann, A. M. (2004). Molecular Identification of the Catabolic Vinyl Chloride Reductase from Dehalococcoides sp. Strain VS and Its Environmental Distribution. Appl. Environ. Microbiol. 70: 4880-4888 [Abstract] [Full Text]  
• Maillard, J., Schumacher, W., Vazquez, F., Regeard, C., Hagen, W. R., Holliger, C. (2003). Characterization of the Corrinoid Iron-Sulfur Protein Tetrachloroethene Reductive Dehalogenase of Dehalobacter restrictus. Appl. Environ. Microbiol. 69: 4628-4638 [Abstract] [Full Text]  
• Ahn, Y.-B., Rhee, S.-K., Fennell, D. E., Kerkhof, L. J., Hentschel, U., Haggblom, M. M. (2003). Reductive Dehalogenation of Brominated Phenolic Compounds by Microorganisms Associated with the Marine Sponge Aplysina aerophoba. Appl. Environ. Microbiol. 69: 4159-4166 [Abstract] [Full Text]  
• Sung, Y., Ritalahti, K. M., Sanford, R. A., Urbance, J. W., Flynn, S. J., Tiedje, J. M., Loffler, F. E. (2003). Characterization of Two Tetrachloroethene-Reducing, Acetate-Oxidizing Anaerobic Bacteria and Their Description as Desulfuromonas michiganensis sp. nov.. Appl. Environ. Microbiol. 69: 2964-2974 [Abstract] [Full Text]